Quantitative proteomics of synaptic and nonsynaptic mitochondria: Insights for synaptic mitochondrial vulnerability

Kelly L. Stauch, Phillip R. Purnell, Howard S Fox

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Synaptic mitochondria are essential for maintaining calcium homeostasis and producing ATP, processes vital for neuronal integrity and synaptic transmission. Synaptic mitochondria exhibit increased oxidative damage during aging and are more vulnerable to calcium insult than nonsynaptic mitochondria. Why synaptic mitochondria are specifically more susceptible to cumulative damage remains to be determined. In this study, the generation of a super-SILAC mix that served as an appropriate internal standard for mouse brain mitochondria mass spectrometry based analysis allowed for the quantification of the proteomic differences between synaptic and nonsynaptic mitochondria isolated from 10-month-old mice. We identified a total of 2260 common proteins between synaptic and nonsynaptic mitochondria of which 1629 were annotated as mitochondrial. Quantitative proteomic analysis of the proteins common between synaptic and nonsynaptic mitochondria revealed significant differential expression of 522 proteins involved in several pathways including oxidative phosphorylation, mitochondrial fission/fusion, calcium transport, and mitochondrial DNA replication and maintenance. In comparison to nonsynaptic mitochondria, synaptic mitochondria exhibited increased age-associated mitochondrial DNA deletions and decreased bioenergetic function. These findings provide insights into synaptic mitochondrial susceptibility to damage.

Original languageEnglish (US)
Pages (from-to)2620-2636
Number of pages17
JournalJournal of proteome research
Volume13
Issue number5
DOIs
StatePublished - May 2 2014

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Mitochondria
Proteomics
Calcium
Mitochondrial DNA
Mitochondrial Dynamics
Proteins
Oxidative Phosphorylation
DNA Replication
Synaptic Transmission
Energy Metabolism
Mass spectrometry
Brain
Mass Spectrometry
Homeostasis
Fusion reactions
Adenosine Triphosphate
Aging of materials
Maintenance

Keywords

  • calcium
  • fission/fusion
  • mitochondria
  • mitochondrial DNA deletions
  • neurons
  • oxidative phosphorylation
  • synapse

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Quantitative proteomics of synaptic and nonsynaptic mitochondria : Insights for synaptic mitochondrial vulnerability. / Stauch, Kelly L.; Purnell, Phillip R.; Fox, Howard S.

In: Journal of proteome research, Vol. 13, No. 5, 02.05.2014, p. 2620-2636.

Research output: Contribution to journalArticle

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