Quantitative Proteomics of Presynaptic Mitochondria Reveal an Overexpression and Biological Relevance of Neuronal MitoNEET in Postnatal Brain Development

Kelly L. Stauch, Lance M. Villeneuve, Steven Totusek, Benjamin Lamberty, Pawel Ciborowski, Howard S. Fox

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although it has been recognized that energy metabolism and mitochondrial structure and functional activity in the immature brain differs from that of the adult, few studies have examined mitochondria specifically at the neuronal synapse during postnatal brain development. In this study, we examined the presynaptic mitochondrial proteome in mice at postnatal day 7 and 42, a period that involves the formation and maturation of synapses. Application of two independent quantitative proteomics approaches – SWATH-MS and super-SILAC – revealed a total of 40 proteins as significantly differentially expressed in the presynaptic mitochondria. In addition to elevated levels of proteins known to be involved in ATP metabolic processes, our results identified increased levels of mitoNEET (Cisd1), an iron-sulfur containing protein that regulates mitochondrial bioenergetics. We found that mitoNEET overexpression plays a cell-type specific role in ATP synthesis and in neuronal cells promotes ATP generation. The elevated ATP levels in SH-SY5Y neuroblastoma cells were associated with increased mitochondrial membrane potential and a fragmented mitochondrial network, further supporting a role for mitoNEET as a key regulator of mitochondrial function.

Original languageEnglish (US)
Pages (from-to)370-386
Number of pages17
JournalDevelopmental Neurobiology
Volume79
Issue number4
DOIs
StatePublished - Apr 2019

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Proteomics
Mitochondria
Adenosine Triphosphate
Brain
Synapses
Energy Metabolism
Iron-Sulfur Proteins
Mitochondrial Membrane Potential
Proteome
Neuroblastoma
Proteins

Keywords

  • mitoNEET
  • mitochondria
  • proteomics
  • synaptic

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Quantitative Proteomics of Presynaptic Mitochondria Reveal an Overexpression and Biological Relevance of Neuronal MitoNEET in Postnatal Brain Development. / Stauch, Kelly L.; Villeneuve, Lance M.; Totusek, Steven; Lamberty, Benjamin; Ciborowski, Pawel; Fox, Howard S.

In: Developmental Neurobiology, Vol. 79, No. 4, 04.2019, p. 370-386.

Research output: Contribution to journalArticle

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