Quantitative proteomics analysis of camelina sativa seeds overexpressing the AGG3 gene to identify the proteomic basis of increased yield and stress tolerance

Sophie Alvarez, Swarup Roy Choudhury, Kumaran Sivagnanam, Leslie M. Hicks, Sona Pandey

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Camelina sativa, a close relative of Arabidopsis, is an oilseed plant that is emerging as an important biofuel resource. The genome and transcriptome maps of Camelina have become available recently, but its proteome composition remained unexplored. A labeling LC-based quantitative proteomics approach was applied to decipher the Camelina seed proteome, which led to the identification of 1532 proteins. In addition, the effect of overexpression of the Arabidopsis G-protein γ subunit 3 (AGG3) on the Camelina seed proteome was elucidated to identify the proteomic basis of its increased seed size and improved stress tolerance. The comparative analysis showed a significantly higher expression of proteins involved in primary and secondary metabolism, nucleic acid and protein metabolism, and abscisic acid related responses, corroborating the physiological effects of AGG3 overexpression. More importantly, the proteomic data suggested involvement of the AGG3 protein in the regulation of oxidative stress and heavy metal stress tolerance. These observations were confirmed by the physiological and biochemical characterization of AGG3-overexpressing seeds, which exhibit a higher tolerance to exogenous cadmium in a glutathione-dependent manner. The activity of multiple redox-regulating enzymes is higher in seeds expressing enhanced levels of AGG3. Overall, these data provide critical evidence for the role of redox regulation by the AGG3 protein in mediating important seed-related traits.

Original languageEnglish (US)
Pages (from-to)2606-2616
Number of pages11
JournalJournal of proteome research
Volume14
Issue number6
DOIs
StatePublished - Jun 5 2015

Fingerprint

Proteomics
Seed
Seeds
Genes
Proteome
Proteins
Oxidation-Reduction
Arabidopsis Proteins
Secondary Metabolism
Oilseeds
Abscisic Acid
Oxidative stress
Biofuels
Protein Subunits
Heavy Metals
Cadmium
Transcriptome
GTP-Binding Proteins
Arabidopsis
Metabolism

Keywords

  • AGG3
  • Camelina sativa
  • iTRAQ
  • quantitative proteomics
  • redox regulation
  • seed proteome

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Quantitative proteomics analysis of camelina sativa seeds overexpressing the AGG3 gene to identify the proteomic basis of increased yield and stress tolerance. / Alvarez, Sophie; Roy Choudhury, Swarup; Sivagnanam, Kumaran; Hicks, Leslie M.; Pandey, Sona.

In: Journal of proteome research, Vol. 14, No. 6, 05.06.2015, p. 2606-2616.

Research output: Contribution to journalArticle

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