Quantitative proteomic analyses of the microbial degradation of estrone under various background nitrogen and carbon conditions

Zhe Du, Yinguang Chen, Xu Li

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microbial degradation of estrogenic compounds can be affected by the nitrogen source and background carbon in the environment. However, the underlying mechanisms are not well understood. The objective of this study was to elucidate the molecular mechanisms of estrone (E1) biodegradation at the protein level under various background nitrogen (nitrate or ammonium) and carbon conditions (no background carbon, acetic acid, or humic acid as background carbon) by a newly isolated bacterial strain. The E1 degrading bacterial strain, Hydrogenophaga atypica ZD1, was isolated from river sediments and its proteome was characterized under various experimental conditions using quantitative proteomics. Results show that the E1 degradation rate was faster when ammonium was used as the nitrogen source than with nitrate. The degradation rate was also faster when either acetic acid or humic acid was present in the background. Proteomics analyses suggested that the E1 biodegradation products enter the tyrosine metabolism pathway. Compared to nitrate, ammonium likely promoted E1 degradation by increasing the activities of the branched-chain-amino-acid aminotransferase (IlvE) and enzymes involved in the glutamine synthetase-glutamine oxoglutarate aminotransferase (GS-GOGAT) pathway. The increased E1 degradation rate with acetic acid or humic acid in the background can also be attributed to the up-regulation of IlvE. Results from this study can help predict and explain E1 biodegradation kinetics under various environmental conditions.

Original languageEnglish (US)
Pages (from-to)361-368
Number of pages8
JournalWater Research
Volume123
DOIs
StatePublished - Jan 1 2017

Fingerprint

proteomics
Nitrogen
Degradation
degradation
Biodegradation
Carbon
Acetic acid
acetic acid
nitrogen
humic acid
carbon
Nitrates
biodegradation
ammonium
nitrate
estrogenic compound
Proteins
ammonium nitrate
Metabolism
fluvial deposit

Keywords

  • Background carbon substrates
  • Degradation pathway
  • Estrone
  • Microbial degradation
  • Nitrogen source
  • Quantitative proteomics

ASJC Scopus subject areas

  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

Quantitative proteomic analyses of the microbial degradation of estrone under various background nitrogen and carbon conditions. / Du, Zhe; Chen, Yinguang; Li, Xu.

In: Water Research, Vol. 123, 01.01.2017, p. 361-368.

Research output: Contribution to journalArticle

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