Quantitative evaluation of synbiotic strategies to improve persistence and metabolic activity of Lactobacillus reuteri DSM 17938 in the human gastrointestinal tract

Monchaya Rattanaprasert, Stefan Roos, Robert W Hutkins, Jens Walter

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The efficacy of three synbiotic approaches to improve persistence and metabolic activity of Lactobacillus reuteri DSM 17938 in the human gut was determined in a single-blind, randomized, crossover, placebo-controlled trial. Synbiotics consisting of 5 × 108 bacterial cells and 2 g of either GOS, rhamnose or the combination of both were given to 15 healthy adults for 7 days, followed by 10 days during which only the carbohydrates were administered. Faecal samples were collected and quantitative real-time PCR was used to determine cell numbers and rRNA copy number. Although L. reuteri DSM 17938 was detectable in faecal samples after consumption, the addition of GOS, rhamnose, and the mixture of the both did not increase faecal populations, nor was persistence enhanced after consumption of the probiotic had ended. However, based on rRNA per cell ratios, the combination of GOS and rhamnose significantly stimulated metabolic activity of the L. reuteri strain.

Original languageEnglish (US)
Pages (from-to)85-94
Number of pages10
JournalJournal of Functional Foods
Volume10
DOIs
StatePublished - Sep 1 2014

Fingerprint

Synbiotics
Lactobacillus reuteri
Rhamnose
rhamnose
gastrointestinal system
dietary supplements
Gastrointestinal Tract
ribosomal RNA
Probiotics
cells
probiotics
placebos
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
digestive system
Cell Count
Placebos
Carbohydrates
carbohydrates
sampling

Keywords

  • 1,2-Propanediol
  • Galactooligosaccharide
  • Lactobacillus reuteri
  • Probiotic
  • Rhamnose
  • Synbiotic

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Nutrition and Dietetics

Cite this

Quantitative evaluation of synbiotic strategies to improve persistence and metabolic activity of Lactobacillus reuteri DSM 17938 in the human gastrointestinal tract. / Rattanaprasert, Monchaya; Roos, Stefan; Hutkins, Robert W; Walter, Jens.

In: Journal of Functional Foods, Vol. 10, 01.09.2014, p. 85-94.

Research output: Contribution to journalArticle

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