Quantitative enzymatic synthesis of 2-acetamido-2-deoxy-α-d-glucopyranosyl-1,6-diphosphate

Pi Wan Cheng, Don M. Carlson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Phosphoacetylglucosamine mutase (EC 2.7.5.2) catalyzes the interconversion of N-acetylglucosamine-1-phosphate and of N-acetylglucosamine-6-phosphate. Glucose-1,6-diphosphate activates this mutase through the formation of N-acetylglucosamine-1,6-diphosphate and glucose-6-phosphate. By adding glucose-6-phosphate dehydrogenase and an excess of N-acetylglucosamine-6-phosphate the reaction is pulled to completion with stoichiometric formation of N-acetylglucosamine-1,6-diphosphate with respect to the amount of glucose-1,6-diphosphate present. The isolation, chemical analysis, molar rotation, and cofactor activity of N-acetylglucosamine-1,6-diphosphate are described.

Original languageEnglish (US)
Pages (from-to)533-540
Number of pages8
JournalAnalytical Biochemistry
Volume85
Issue number2
DOIs
StatePublished - Apr 1978

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Acetylglucosamine
Diphosphates
Intramolecular Transferases
Glucose-6-Phosphate
Glucosephosphate Dehydrogenase
Chemical analysis
glucose-1,6-bisphosphate
N-acetylglucosamine 6-phosphate

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Quantitative enzymatic synthesis of 2-acetamido-2-deoxy-α-d-glucopyranosyl-1,6-diphosphate. / Cheng, Pi Wan; Carlson, Don M.

In: Analytical Biochemistry, Vol. 85, No. 2, 04.1978, p. 533-540.

Research output: Contribution to journalArticle

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N2 - Phosphoacetylglucosamine mutase (EC 2.7.5.2) catalyzes the interconversion of N-acetylglucosamine-1-phosphate and of N-acetylglucosamine-6-phosphate. Glucose-1,6-diphosphate activates this mutase through the formation of N-acetylglucosamine-1,6-diphosphate and glucose-6-phosphate. By adding glucose-6-phosphate dehydrogenase and an excess of N-acetylglucosamine-6-phosphate the reaction is pulled to completion with stoichiometric formation of N-acetylglucosamine-1,6-diphosphate with respect to the amount of glucose-1,6-diphosphate present. The isolation, chemical analysis, molar rotation, and cofactor activity of N-acetylglucosamine-1,6-diphosphate are described.

AB - Phosphoacetylglucosamine mutase (EC 2.7.5.2) catalyzes the interconversion of N-acetylglucosamine-1-phosphate and of N-acetylglucosamine-6-phosphate. Glucose-1,6-diphosphate activates this mutase through the formation of N-acetylglucosamine-1,6-diphosphate and glucose-6-phosphate. By adding glucose-6-phosphate dehydrogenase and an excess of N-acetylglucosamine-6-phosphate the reaction is pulled to completion with stoichiometric formation of N-acetylglucosamine-1,6-diphosphate with respect to the amount of glucose-1,6-diphosphate present. The isolation, chemical analysis, molar rotation, and cofactor activity of N-acetylglucosamine-1,6-diphosphate are described.

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