Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background

Shobana Raghunath, Abhijeet Prasad, Rahul Tevatia, Jillian R. Gunther, Santanu Roy, Sunil Krishnan, Ravi F Saraf

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100 % in a mixture with five nonspecific miRNA each with a 103-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10−2 to 103 femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 103 copies of a miRNA sequence in RNA extracted from 100 μL of plasma.

Original languageEnglish (US)
Pages (from-to)429-433
Number of pages5
JournalChemElectroChem
Volume5
Issue number3
DOIs
StatePublished - Feb 2018

Fingerprint

Microarrays
MicroRNAs
DNA
Plasmas
Electrodes
Biomarkers
RNA
Multiplexing
Labels
Blood
Complementary DNA

Keywords

  • DNA sequencing
  • blood test
  • cancer diagnosis
  • electrochemical analysis
  • microRNA
  • microarray

ASJC Scopus subject areas

  • Catalysis
  • Electrochemistry

Cite this

Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background. / Raghunath, Shobana; Prasad, Abhijeet; Tevatia, Rahul; Gunther, Jillian R.; Roy, Santanu; Krishnan, Sunil; Saraf, Ravi F.

In: ChemElectroChem, Vol. 5, No. 3, 02.2018, p. 429-433.

Research output: Contribution to journalArticle

Raghunath, Shobana ; Prasad, Abhijeet ; Tevatia, Rahul ; Gunther, Jillian R. ; Roy, Santanu ; Krishnan, Sunil ; Saraf, Ravi F. / Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background. In: ChemElectroChem. 2018 ; Vol. 5, No. 3. pp. 429-433.
@article{e139cbc424914014a32f9c4e98622f09,
title = "Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 {\%} Specificity, and Zero Background",
abstract = "Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100 {\%} in a mixture with five nonspecific miRNA each with a 103-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10−2 to 103 femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 103 copies of a miRNA sequence in RNA extracted from 100 μL of plasma.",
keywords = "DNA sequencing, blood test, cancer diagnosis, electrochemical analysis, microRNA, microarray",
author = "Shobana Raghunath and Abhijeet Prasad and Rahul Tevatia and Gunther, {Jillian R.} and Santanu Roy and Sunil Krishnan and Saraf, {Ravi F}",
year = "2018",
month = "2",
doi = "10.1002/celc.201700983",
language = "English (US)",
volume = "5",
pages = "429--433",
journal = "ChemElectroChem",
issn = "2196-0216",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background

AU - Raghunath, Shobana

AU - Prasad, Abhijeet

AU - Tevatia, Rahul

AU - Gunther, Jillian R.

AU - Roy, Santanu

AU - Krishnan, Sunil

AU - Saraf, Ravi F

PY - 2018/2

Y1 - 2018/2

N2 - Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100 % in a mixture with five nonspecific miRNA each with a 103-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10−2 to 103 femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 103 copies of a miRNA sequence in RNA extracted from 100 μL of plasma.

AB - Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100 % in a mixture with five nonspecific miRNA each with a 103-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10−2 to 103 femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 103 copies of a miRNA sequence in RNA extracted from 100 μL of plasma.

KW - DNA sequencing

KW - blood test

KW - cancer diagnosis

KW - electrochemical analysis

KW - microRNA

KW - microarray

UR - http://www.scopus.com/inward/record.url?scp=85038035983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85038035983&partnerID=8YFLogxK

U2 - 10.1002/celc.201700983

DO - 10.1002/celc.201700983

M3 - Article

C2 - 31157159

AN - SCOPUS:85038035983

VL - 5

SP - 429

EP - 433

JO - ChemElectroChem

JF - ChemElectroChem

SN - 2196-0216

IS - 3

ER -