Quantitative assessment of neural outgrowth using spatial light interference microscopy

Young Jae Lee, Pati Cintora, Jyothi Arikkath, Olaoluwa Akinsola, Mikhail Kandel, Gabriel Popescu, Catherine Best-Popescu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Optimal growth as well as branching of axons and dendrites is critical for the nervous system function. Neuritic length, arborization, and growth rate determine the innervation properties of neurons and define each cell's computational capability. Thus, to investigate the nervous system function, we need to develop methods and instrumentation techniques capable of quantifying various aspects of neural network formation: Neuron process extension, retraction, stability, and branching. During the last three decades, fluorescence microscopy has yielded enormous advances in our understanding of neurobiology. While fluorescent markers provide valuable specificity to imaging, photobleaching, and photoxicity often limit the duration of the investigation. Here, we used spatial light interference microscopy (SLIM) to measure quantitatively neurite outgrowth as a function of cell confluence. Because it is label-free and nondestructive, SLIM allows for long-term investigation over many hours. We found that neurons exhibit a higher growth rate of neurite length in low-confluence versus mediumand high-confluence conditions. We believe this methodology will aid investigators in performing unbiased, nondestructive analysis of morphometric neuronal parameters.

Original languageEnglish (US)
Article number066015
JournalJournal of Biomedical Optics
Volume22
Issue number6
DOIs
StatePublished - Jun 1 2017

Fingerprint

Light interference
neurons
Neurons
nervous system
Microscopic examination
Neurology
microscopy
interference
axons
Photobleaching
Fluorescence microscopy
dendrites
cells
markers
Labels
methodology
Neural networks
Imaging techniques
fluorescence

Keywords

  • Quantitative image analysis
  • neurite outgrowth
  • neuroinformatics

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Quantitative assessment of neural outgrowth using spatial light interference microscopy. / Lee, Young Jae; Cintora, Pati; Arikkath, Jyothi; Akinsola, Olaoluwa; Kandel, Mikhail; Popescu, Gabriel; Best-Popescu, Catherine.

In: Journal of Biomedical Optics, Vol. 22, No. 6, 066015, 01.06.2017.

Research output: Contribution to journalArticle

Lee, Young Jae ; Cintora, Pati ; Arikkath, Jyothi ; Akinsola, Olaoluwa ; Kandel, Mikhail ; Popescu, Gabriel ; Best-Popescu, Catherine. / Quantitative assessment of neural outgrowth using spatial light interference microscopy. In: Journal of Biomedical Optics. 2017 ; Vol. 22, No. 6.
@article{197a2ec6ba134f3aa34fdc9ec2e2a947,
title = "Quantitative assessment of neural outgrowth using spatial light interference microscopy",
abstract = "Optimal growth as well as branching of axons and dendrites is critical for the nervous system function. Neuritic length, arborization, and growth rate determine the innervation properties of neurons and define each cell's computational capability. Thus, to investigate the nervous system function, we need to develop methods and instrumentation techniques capable of quantifying various aspects of neural network formation: Neuron process extension, retraction, stability, and branching. During the last three decades, fluorescence microscopy has yielded enormous advances in our understanding of neurobiology. While fluorescent markers provide valuable specificity to imaging, photobleaching, and photoxicity often limit the duration of the investigation. Here, we used spatial light interference microscopy (SLIM) to measure quantitatively neurite outgrowth as a function of cell confluence. Because it is label-free and nondestructive, SLIM allows for long-term investigation over many hours. We found that neurons exhibit a higher growth rate of neurite length in low-confluence versus mediumand high-confluence conditions. We believe this methodology will aid investigators in performing unbiased, nondestructive analysis of morphometric neuronal parameters.",
keywords = "Quantitative image analysis, neurite outgrowth, neuroinformatics",
author = "Lee, {Young Jae} and Pati Cintora and Jyothi Arikkath and Olaoluwa Akinsola and Mikhail Kandel and Gabriel Popescu and Catherine Best-Popescu",
year = "2017",
month = "6",
day = "1",
doi = "10.1117/1.JBO.22.6.066015",
language = "English (US)",
volume = "22",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "6",

}

TY - JOUR

T1 - Quantitative assessment of neural outgrowth using spatial light interference microscopy

AU - Lee, Young Jae

AU - Cintora, Pati

AU - Arikkath, Jyothi

AU - Akinsola, Olaoluwa

AU - Kandel, Mikhail

AU - Popescu, Gabriel

AU - Best-Popescu, Catherine

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Optimal growth as well as branching of axons and dendrites is critical for the nervous system function. Neuritic length, arborization, and growth rate determine the innervation properties of neurons and define each cell's computational capability. Thus, to investigate the nervous system function, we need to develop methods and instrumentation techniques capable of quantifying various aspects of neural network formation: Neuron process extension, retraction, stability, and branching. During the last three decades, fluorescence microscopy has yielded enormous advances in our understanding of neurobiology. While fluorescent markers provide valuable specificity to imaging, photobleaching, and photoxicity often limit the duration of the investigation. Here, we used spatial light interference microscopy (SLIM) to measure quantitatively neurite outgrowth as a function of cell confluence. Because it is label-free and nondestructive, SLIM allows for long-term investigation over many hours. We found that neurons exhibit a higher growth rate of neurite length in low-confluence versus mediumand high-confluence conditions. We believe this methodology will aid investigators in performing unbiased, nondestructive analysis of morphometric neuronal parameters.

AB - Optimal growth as well as branching of axons and dendrites is critical for the nervous system function. Neuritic length, arborization, and growth rate determine the innervation properties of neurons and define each cell's computational capability. Thus, to investigate the nervous system function, we need to develop methods and instrumentation techniques capable of quantifying various aspects of neural network formation: Neuron process extension, retraction, stability, and branching. During the last three decades, fluorescence microscopy has yielded enormous advances in our understanding of neurobiology. While fluorescent markers provide valuable specificity to imaging, photobleaching, and photoxicity often limit the duration of the investigation. Here, we used spatial light interference microscopy (SLIM) to measure quantitatively neurite outgrowth as a function of cell confluence. Because it is label-free and nondestructive, SLIM allows for long-term investigation over many hours. We found that neurons exhibit a higher growth rate of neurite length in low-confluence versus mediumand high-confluence conditions. We believe this methodology will aid investigators in performing unbiased, nondestructive analysis of morphometric neuronal parameters.

KW - Quantitative image analysis

KW - neurite outgrowth

KW - neuroinformatics

UR - http://www.scopus.com/inward/record.url?scp=85021710886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021710886&partnerID=8YFLogxK

U2 - 10.1117/1.JBO.22.6.066015

DO - 10.1117/1.JBO.22.6.066015

M3 - Article

C2 - 28655053

AN - SCOPUS:85021710886

VL - 22

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 6

M1 - 066015

ER -