Quantitative Analysis of Retinal Vessel Changes in Galactose-Fed Dogs

Yukio Takahashi, Wisly Augustin, Milton Wyman, Peter F Kador

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Retinal vessel changes in 36-month 30% galactose-fed beagles treated with or without aldose reductase inhibitors were quantified using an Olympus Cue-3 color image analysis system. Individual maps of the intact retinal vasculature, isolated by trypsin-digestion, were divided into 24 distinct subregions and measurements of either the endothelial cell to pericyte (E/P) ratio or cell densities, expressed as pericytes per mm capillary length or endothelial cells per mm capillary length, were conducted in 0.1 mm2 areas surrounding the midpoints of 12 subregions associated with the highest incidence of microaneurysms. Significantly increased E/P ratios and decreased pericyte densities were observed with the duration of galactose-feeding. These retinal changes were reduced by aldose reductase inhibitor treatment. Correlations between the E/P ratio and either number of microaneurysms or cataract severity were also observed. These data support the dose-dependent effects of aldose reductase inhibitors in preventing pericyte degeneration and subsequent formation of microaneurysms (Archives Ophthalmol. 108:1301, 1990).

Original languageEnglish (US)
Pages (from-to)257-269
Number of pages13
JournalJournal of Ocular Pharmacology
Volume9
Issue number3
DOIs
StatePublished - Jan 1 1993

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Retinal Vessels
Pericytes
Galactose
Aldehyde Reductase
Dogs
Endothelial Cells
Trypsin
Cataract
Cues
Digestion
Color
Cell Count
Incidence
Microaneurysm

ASJC Scopus subject areas

  • Ophthalmology
  • Pharmacology
  • Pharmacology (medical)

Cite this

Quantitative Analysis of Retinal Vessel Changes in Galactose-Fed Dogs. / Takahashi, Yukio; Augustin, Wisly; Wyman, Milton; Kador, Peter F.

In: Journal of Ocular Pharmacology, Vol. 9, No. 3, 01.01.1993, p. 257-269.

Research output: Contribution to journalArticle

Takahashi, Yukio ; Augustin, Wisly ; Wyman, Milton ; Kador, Peter F. / Quantitative Analysis of Retinal Vessel Changes in Galactose-Fed Dogs. In: Journal of Ocular Pharmacology. 1993 ; Vol. 9, No. 3. pp. 257-269.
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