Quantitative analysis of human keratinocyte cell elasticity using atomic force microscopy (AFM)

Carmen Kar Man Fung, Ning Xi, Ruiguo Yang, Kristina Seiffert-Sinha, King Wai Chiu Lai, Animesh A. Sinha

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We present the use of atomic force microscopy (AFM) to visualize and quantify the dynamics of epithelial cell junction interactions under physiological and pathophysiological conditions at the nanoscale. Desmosomal junctions are critical cellular adhesion components within epithelial tissues and blistering skin diseases such as Pemphigus are the result in the disruption of these components. However, these structures are complex and mechanically inhomogeneous, making them difficult to study. The mechanisms of autoantibody mediated keratinocyte disassembly remain largely unknown. Here, we have used AFM technology to image and measure the mechanical properties of living skin epithelial cells in culture. We demonstrate that force measurement data can distinguish cells cultured with and without autoantibody treatment. Our demonstration of the use of AFM for in situ imaging and elasticity measurements at the local, or tissue level opens potential new avenues for the investigation of disease mechanisms and monitoring of therapeutic strategies in blistering skin diseases.

Original languageEnglish (US)
Article number5720318
Pages (from-to)9-15
Number of pages7
JournalIEEE Transactions on Nanobioscience
Volume10
Issue number1
DOIs
StatePublished - Mar 1 2011

Fingerprint

Atomic Force Microscopy
Elasticity
Keratinocytes
Atomic force microscopy
Skin
Skin Diseases
Autoantibodies
Epithelial Cells
Chemical analysis
Tissue
Intercellular Junctions
Pemphigus
Force measurement
Cell Communication
Cultured Cells
Demonstrations
Adhesion
Epithelium
Cell Culture Techniques
Technology

Keywords

  • AFM
  • cell elasticity
  • cell junctions
  • desmosome
  • human keratinocyte

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

Quantitative analysis of human keratinocyte cell elasticity using atomic force microscopy (AFM). / Fung, Carmen Kar Man; Xi, Ning; Yang, Ruiguo; Seiffert-Sinha, Kristina; Lai, King Wai Chiu; Sinha, Animesh A.

In: IEEE Transactions on Nanobioscience, Vol. 10, No. 1, 5720318, 01.03.2011, p. 9-15.

Research output: Contribution to journalArticle

Fung, Carmen Kar Man ; Xi, Ning ; Yang, Ruiguo ; Seiffert-Sinha, Kristina ; Lai, King Wai Chiu ; Sinha, Animesh A. / Quantitative analysis of human keratinocyte cell elasticity using atomic force microscopy (AFM). In: IEEE Transactions on Nanobioscience. 2011 ; Vol. 10, No. 1. pp. 9-15.
@article{77cb9e47be67456daeb30e97db59bfed,
title = "Quantitative analysis of human keratinocyte cell elasticity using atomic force microscopy (AFM)",
abstract = "We present the use of atomic force microscopy (AFM) to visualize and quantify the dynamics of epithelial cell junction interactions under physiological and pathophysiological conditions at the nanoscale. Desmosomal junctions are critical cellular adhesion components within epithelial tissues and blistering skin diseases such as Pemphigus are the result in the disruption of these components. However, these structures are complex and mechanically inhomogeneous, making them difficult to study. The mechanisms of autoantibody mediated keratinocyte disassembly remain largely unknown. Here, we have used AFM technology to image and measure the mechanical properties of living skin epithelial cells in culture. We demonstrate that force measurement data can distinguish cells cultured with and without autoantibody treatment. Our demonstration of the use of AFM for in situ imaging and elasticity measurements at the local, or tissue level opens potential new avenues for the investigation of disease mechanisms and monitoring of therapeutic strategies in blistering skin diseases.",
keywords = "AFM, cell elasticity, cell junctions, desmosome, human keratinocyte",
author = "Fung, {Carmen Kar Man} and Ning Xi and Ruiguo Yang and Kristina Seiffert-Sinha and Lai, {King Wai Chiu} and Sinha, {Animesh A.}",
year = "2011",
month = "3",
day = "1",
doi = "10.1109/TNB.2011.2113397",
language = "English (US)",
volume = "10",
pages = "9--15",
journal = "IEEE Transactions on Nanobioscience",
issn = "1536-1241",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
number = "1",

}

TY - JOUR

T1 - Quantitative analysis of human keratinocyte cell elasticity using atomic force microscopy (AFM)

AU - Fung, Carmen Kar Man

AU - Xi, Ning

AU - Yang, Ruiguo

AU - Seiffert-Sinha, Kristina

AU - Lai, King Wai Chiu

AU - Sinha, Animesh A.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - We present the use of atomic force microscopy (AFM) to visualize and quantify the dynamics of epithelial cell junction interactions under physiological and pathophysiological conditions at the nanoscale. Desmosomal junctions are critical cellular adhesion components within epithelial tissues and blistering skin diseases such as Pemphigus are the result in the disruption of these components. However, these structures are complex and mechanically inhomogeneous, making them difficult to study. The mechanisms of autoantibody mediated keratinocyte disassembly remain largely unknown. Here, we have used AFM technology to image and measure the mechanical properties of living skin epithelial cells in culture. We demonstrate that force measurement data can distinguish cells cultured with and without autoantibody treatment. Our demonstration of the use of AFM for in situ imaging and elasticity measurements at the local, or tissue level opens potential new avenues for the investigation of disease mechanisms and monitoring of therapeutic strategies in blistering skin diseases.

AB - We present the use of atomic force microscopy (AFM) to visualize and quantify the dynamics of epithelial cell junction interactions under physiological and pathophysiological conditions at the nanoscale. Desmosomal junctions are critical cellular adhesion components within epithelial tissues and blistering skin diseases such as Pemphigus are the result in the disruption of these components. However, these structures are complex and mechanically inhomogeneous, making them difficult to study. The mechanisms of autoantibody mediated keratinocyte disassembly remain largely unknown. Here, we have used AFM technology to image and measure the mechanical properties of living skin epithelial cells in culture. We demonstrate that force measurement data can distinguish cells cultured with and without autoantibody treatment. Our demonstration of the use of AFM for in situ imaging and elasticity measurements at the local, or tissue level opens potential new avenues for the investigation of disease mechanisms and monitoring of therapeutic strategies in blistering skin diseases.

KW - AFM

KW - cell elasticity

KW - cell junctions

KW - desmosome

KW - human keratinocyte

UR - http://www.scopus.com/inward/record.url?scp=79955579952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955579952&partnerID=8YFLogxK

U2 - 10.1109/TNB.2011.2113397

DO - 10.1109/TNB.2011.2113397

M3 - Article

VL - 10

SP - 9

EP - 15

JO - IEEE Transactions on Nanobioscience

JF - IEEE Transactions on Nanobioscience

SN - 1536-1241

IS - 1

M1 - 5720318

ER -