Quantitation of uracil in rodent diet

Raymond A. Smith, T. Scott Tibbels, Teresa E. Smith, Samuel Monroe Cohen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Feeding of high levels of uracil to laboratory rodents results in the formation of calculi in the lumen of the urinary bladder. This urolithiasis stimulates cellular proliferation in the bladder and has been used in studies of two-stage carcinogenesis. Quantitation of uracil in rodent diet was achieved by extraction from the diet with ammonium hydroxide. The extract was applied to a strong anion-exchange solid-phase extraction column. Uracil is not retained on this matrix which adsorbs the majority of contaminants in the extract. The uracil was quantitated by HPLC on an ODS microbore column (100 × 2 mm internal diameter) eluted at 0.5 ml/min with 200 mm KH2PO4, pH 3.5, at 30°C. Three structurally related pyrimidine bases, cytosine, uracil, and thymine, showed increasing retention on this column/solvent combination, thereby demonstrating selectivity of the analysis. Recovery of uracil was 76-90% with lower values observed when dietary levels of uracil were in excess of 4.5%.

Original languageEnglish (US)
Pages (from-to)375-377
Number of pages3
JournalAnalytical Biochemistry
Volume195
Issue number2
DOIs
StatePublished - Jan 1 1991

Fingerprint

Uracil
Nutrition
Rodentia
Diet
Urinary Bladder
Ammonium Hydroxide
Urolithiasis
Thymine
Cytosine
Solid Phase Extraction
Calculi
Anions
Ion exchange
Carcinogenesis
High Pressure Liquid Chromatography
Cell Proliferation
Impurities
Recovery

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Quantitation of uracil in rodent diet. / Smith, Raymond A.; Tibbels, T. Scott; Smith, Teresa E.; Cohen, Samuel Monroe.

In: Analytical Biochemistry, Vol. 195, No. 2, 01.01.1991, p. 375-377.

Research output: Contribution to journalArticle

Smith, Raymond A. ; Tibbels, T. Scott ; Smith, Teresa E. ; Cohen, Samuel Monroe. / Quantitation of uracil in rodent diet. In: Analytical Biochemistry. 1991 ; Vol. 195, No. 2. pp. 375-377.
@article{ace3231a9ac445298d5f47f0649f403d,
title = "Quantitation of uracil in rodent diet",
abstract = "Feeding of high levels of uracil to laboratory rodents results in the formation of calculi in the lumen of the urinary bladder. This urolithiasis stimulates cellular proliferation in the bladder and has been used in studies of two-stage carcinogenesis. Quantitation of uracil in rodent diet was achieved by extraction from the diet with ammonium hydroxide. The extract was applied to a strong anion-exchange solid-phase extraction column. Uracil is not retained on this matrix which adsorbs the majority of contaminants in the extract. The uracil was quantitated by HPLC on an ODS microbore column (100 × 2 mm internal diameter) eluted at 0.5 ml/min with 200 mm KH2PO4, pH 3.5, at 30°C. Three structurally related pyrimidine bases, cytosine, uracil, and thymine, showed increasing retention on this column/solvent combination, thereby demonstrating selectivity of the analysis. Recovery of uracil was 76-90{\%} with lower values observed when dietary levels of uracil were in excess of 4.5{\%}.",
author = "Smith, {Raymond A.} and Tibbels, {T. Scott} and Smith, {Teresa E.} and Cohen, {Samuel Monroe}",
year = "1991",
month = "1",
day = "1",
doi = "10.1016/0003-2697(91)90344-S",
language = "English (US)",
volume = "195",
pages = "375--377",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Quantitation of uracil in rodent diet

AU - Smith, Raymond A.

AU - Tibbels, T. Scott

AU - Smith, Teresa E.

AU - Cohen, Samuel Monroe

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Feeding of high levels of uracil to laboratory rodents results in the formation of calculi in the lumen of the urinary bladder. This urolithiasis stimulates cellular proliferation in the bladder and has been used in studies of two-stage carcinogenesis. Quantitation of uracil in rodent diet was achieved by extraction from the diet with ammonium hydroxide. The extract was applied to a strong anion-exchange solid-phase extraction column. Uracil is not retained on this matrix which adsorbs the majority of contaminants in the extract. The uracil was quantitated by HPLC on an ODS microbore column (100 × 2 mm internal diameter) eluted at 0.5 ml/min with 200 mm KH2PO4, pH 3.5, at 30°C. Three structurally related pyrimidine bases, cytosine, uracil, and thymine, showed increasing retention on this column/solvent combination, thereby demonstrating selectivity of the analysis. Recovery of uracil was 76-90% with lower values observed when dietary levels of uracil were in excess of 4.5%.

AB - Feeding of high levels of uracil to laboratory rodents results in the formation of calculi in the lumen of the urinary bladder. This urolithiasis stimulates cellular proliferation in the bladder and has been used in studies of two-stage carcinogenesis. Quantitation of uracil in rodent diet was achieved by extraction from the diet with ammonium hydroxide. The extract was applied to a strong anion-exchange solid-phase extraction column. Uracil is not retained on this matrix which adsorbs the majority of contaminants in the extract. The uracil was quantitated by HPLC on an ODS microbore column (100 × 2 mm internal diameter) eluted at 0.5 ml/min with 200 mm KH2PO4, pH 3.5, at 30°C. Three structurally related pyrimidine bases, cytosine, uracil, and thymine, showed increasing retention on this column/solvent combination, thereby demonstrating selectivity of the analysis. Recovery of uracil was 76-90% with lower values observed when dietary levels of uracil were in excess of 4.5%.

UR - http://www.scopus.com/inward/record.url?scp=0025806585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025806585&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(91)90344-S

DO - 10.1016/0003-2697(91)90344-S

M3 - Article

VL - 195

SP - 375

EP - 377

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -