Quantitation of radiolabeled mucous glycoproteins secreted by tracheal explants

Pi-Wan Cheng, James M. Sherman, Thomas F. Boat, Margaret Bruce

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

An agarose gel column filtration method has been developed to quantitate labeled mucous glycoprotein secreted into culture medium by explants of tracheal epithelium. A multichannel pump is employed to simultaneously analyze 15 samples. Compared to other methods, such as dialysis and trichloroacetic acid/phosphotungstic acid precipitation methods, the column method not only quantitates high-molecular-weight mucous glycoprotein specifically but also offers the advantages of rapid processing of samples, low-background radioactivity, linearity and reproducibility even with small amounts of labeled mucous glycoproteins, and recovery of samples for chemical analysis. This method can be used to quantitate any substances that can be isolated by a one-step column chromatographic technique.

Original languageEnglish (US)
Pages (from-to)301-306
Number of pages6
JournalAnalytical Biochemistry
Volume117
Issue number2
DOIs
StatePublished - Nov 1 1981

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Glycoproteins
Phosphotungstic Acid
Trichloroacetic Acid
Dialysis
Radioactivity
Sepharose
Culture Media
Gels
Molecular weight
Pumps
Recovery
Gel Chromatography
Processing
Chemical analysis
Epithelium
Molecular Weight

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Quantitation of radiolabeled mucous glycoproteins secreted by tracheal explants. / Cheng, Pi-Wan; Sherman, James M.; Boat, Thomas F.; Bruce, Margaret.

In: Analytical Biochemistry, Vol. 117, No. 2, 01.11.1981, p. 301-306.

Research output: Contribution to journalArticle

Cheng, Pi-Wan ; Sherman, James M. ; Boat, Thomas F. ; Bruce, Margaret. / Quantitation of radiolabeled mucous glycoproteins secreted by tracheal explants. In: Analytical Biochemistry. 1981 ; Vol. 117, No. 2. pp. 301-306.
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