Quantification of eicosanoids and their metabolites in biological matrices: A review

Yashpal S. Chhonker, Veenu Bala, Daryl J Murry

Research output: Contribution to journalReview article

2 Scopus citations

Abstract

The quantification of eicosanoids and their metabolites in biological samples remain an analytical challenge, even though a number of methodologies/techniques have been developed. The major difficulties encountered are related to the oxidation of eicosanoids and their low quantities in biological matrices. Among the known methodologies, liquid chromatography-mass spectrometry (LC-MS/MS) is the standard method for eicosanoid quantification in biological samples. Recently advances have improved the ability to identify and simultaneous quantitate eicosanoids in biological matrices. The present article reviews the quantitative analysis of eicosanoids in different biological matrices by LC and ultra performance liquid chromatography (UPLC)-MS/MS and discusses important aspects to be considered during the collection, sample preparation and the generation of calibration curves required for eicosanoid analysis.

Original languageEnglish (US)
Pages (from-to)2027-2046
Number of pages20
JournalBioanalysis
Volume10
Issue number24
DOIs
Publication statusPublished - Dec 2018

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Keywords

  • LC and UPLC-MS/MS
  • biomarker
  • biomatrices
  • calibration
  • eicosanoids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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