Qualitative Profiling of the Humoral Immune Response Elicited by rVSV-ΔG-EBOV-GP Using a Systems Serology Assay, Domain Programmable Arrays

Mariano Sanchez-Lockhart, Daniel S. Reyes, Jeanette C. Gonzalez, Karla Y. Garcia, Erika C. Villa, Bradley P. Pfeffer, John C. Trefry, Jeffrey R. Kugelman, Margaret L. Pitt, Gustavo F. Palacios

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Sanchez-Lockhart et al. describe a systems serology platform (Domain Programmable Arrays) to identify epitopes targeted after vaccination or infection. They characterize the humoral response in rVSV-ΔG-EBOV-glycoprotein-vaccinated non-human primates, observing bias on the immunoglobulin isotype epitope recognition pattern, showing the value of the tool to qualitatively measure humoral responses.

Original languageEnglish (US)
Pages (from-to)1050-1059.e5
JournalCell Reports
Volume24
Issue number4
DOIs
StatePublished - Jul 24 2018

Fingerprint

Serology
Humoral Immunity
Epitopes
Assays
Immunoglobulin Isotypes
Primates
Pattern recognition
Glycoproteins
Vaccination
Infection

Keywords

  • Domain Programmable Arrays
  • EBOV
  • G-EBOV-GP
  • epitope mapping
  • humoral response
  • rVSV-Δ
  • system serology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Qualitative Profiling of the Humoral Immune Response Elicited by rVSV-ΔG-EBOV-GP Using a Systems Serology Assay, Domain Programmable Arrays. / Sanchez-Lockhart, Mariano; Reyes, Daniel S.; Gonzalez, Jeanette C.; Garcia, Karla Y.; Villa, Erika C.; Pfeffer, Bradley P.; Trefry, John C.; Kugelman, Jeffrey R.; Pitt, Margaret L.; Palacios, Gustavo F.

In: Cell Reports, Vol. 24, No. 4, 24.07.2018, p. 1050-1059.e5.

Research output: Contribution to journalArticle

Sanchez-Lockhart, M, Reyes, DS, Gonzalez, JC, Garcia, KY, Villa, EC, Pfeffer, BP, Trefry, JC, Kugelman, JR, Pitt, ML & Palacios, GF 2018, 'Qualitative Profiling of the Humoral Immune Response Elicited by rVSV-ΔG-EBOV-GP Using a Systems Serology Assay, Domain Programmable Arrays', Cell Reports, vol. 24, no. 4, pp. 1050-1059.e5. https://doi.org/10.1016/j.celrep.2018.06.077
Sanchez-Lockhart, Mariano ; Reyes, Daniel S. ; Gonzalez, Jeanette C. ; Garcia, Karla Y. ; Villa, Erika C. ; Pfeffer, Bradley P. ; Trefry, John C. ; Kugelman, Jeffrey R. ; Pitt, Margaret L. ; Palacios, Gustavo F. / Qualitative Profiling of the Humoral Immune Response Elicited by rVSV-ΔG-EBOV-GP Using a Systems Serology Assay, Domain Programmable Arrays. In: Cell Reports. 2018 ; Vol. 24, No. 4. pp. 1050-1059.e5.
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