Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in Quality Protein Maize

Xiaomei Guo, Kyla Ronhovde, Lingling Yuan, Bo Yao, Madhavan P. Soundararajan, Thomas Elthon, Chi Zhang, David R. Holding

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Quality Protein Maize (QPM) is a hard-endosperm version of the high-lysine opaque2 (o2) maize (Zea mays) mutant, but the genes involved in modification of the soft o2 endosperm are largely unknown. Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase (PFP) catalyzes the ATP-independent conversion of fructose-6-phosphate to fructose-1,6-bisphosphate in glycolysis. We found a large increase in transcript and protein levels of the a-regulatory subunit of PFP (PFPa) in QPM endosperm. In vitro enzyme assays showed a significant increase in forward PFP activity in developing endosperm extracts of QPM relative to the wild type and o2. An expressed retrogene version of PFPa of unknown function that was not up-regulated in QPM was also identified. The elevated expression levels of a number of ATP-requiring heat shock proteins (Hsps) in o2 endosperm are ameliorated in QPM. PFPa is also coinduced with Hsps in maize roots in response to heat, cold, and the unfolded protein response stresses. We propose that reduced ATP availability resulting from the generalized Hsp response in addition to the reduction of pyruvate, orthophosphate dikinase activity in o2 endosperm is compensated in part by increased PFP activity in QPM.

Original languageEnglish (US)
Pages (from-to)917-929
Number of pages13
JournalPlant physiology
Volume158
Issue number2
DOIs
StatePublished - Feb 1 2012

Fingerprint

pyrophosphate-fructose 6-phosphate 1-phosphotransferase
6-phosphofructokinase
Endosperm
pyrophosphates
endosperm
Zea mays
Gene Expression
gene expression
corn
Proteins
proteins
Adenosine Triphosphate
heat shock proteins
Heat-Shock Proteins
Orthophosphate Dikinase Pyruvate
unfolded protein response
fructose 6-phosphate
Unfolded Protein Response
glycolysis
Enzyme Assays

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in Quality Protein Maize. / Guo, Xiaomei; Ronhovde, Kyla; Yuan, Lingling; Yao, Bo; Soundararajan, Madhavan P.; Elthon, Thomas; Zhang, Chi; Holding, David R.

In: Plant physiology, Vol. 158, No. 2, 01.02.2012, p. 917-929.

Research output: Contribution to journalArticle

Guo, Xiaomei ; Ronhovde, Kyla ; Yuan, Lingling ; Yao, Bo ; Soundararajan, Madhavan P. ; Elthon, Thomas ; Zhang, Chi ; Holding, David R. / Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in Quality Protein Maize. In: Plant physiology. 2012 ; Vol. 158, No. 2. pp. 917-929.
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