Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum 'Synergistetes' isolated from the human oral cavity

Julia Downes, Sonia R. Vartoukian, Floyd E. Dewhirst, Jacques Izard, Tsute Chen, Wen Han Yu, Iain C. Sutcliffe, William G. Wade

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Four strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group distinct from any species with validly published names. 16S rRNA and 23S rRNA gene sequence analyses and DNA-DNA reassociation data revealed that the strains constituted a novel group within the phylum 'Synergistetes' and were most closely related to Jonquetella anthropi. Two libraries of randomly cloned DNA were prepared from strain W5455T and were sequenced to provide a genome survey as a resource for metagenomic studies. A new genus and novel species, Pyramidobacter piscolens gen. nov., sp. nov., is proposed to accommodate these strains. The genus Pyramidobacter comprises strains that are anaerobic, non-motile, asaccharolytic bacilli that produce acetic and isovaleric acids and minor to trace amounts of propionic, isobutyric, succinic and phenylacetic acids as end products of metabolism. P. piscolens gen. nov., sp. nov. produced hydrogen sulphide but was otherwise largely biochemically unreactive. Growth was stimulated by the addition of glycine to broth media. The G+C content of the DNA of the type strain was 59 mol%. The type strain of Pyramidobacter piscolens sp. nov. is W5455T (=DSM 21147T=CCUG 55836T).

Original languageEnglish (US)
Pages (from-to)972-980
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume59
Issue number5
DOIs
StatePublished - Nov 10 2009

Fingerprint

Synergistetes
Mouth
mouth
cavity
DNA
Bacillus
new species
Phenylacetates
Isobutyrates
Metagenomics
Hydrogen Sulfide
Propionates
Base Composition
Succinic Acid
DNA Sequence Analysis
rRNA Genes
Glycine
Libraries
Names
acid

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum 'Synergistetes' isolated from the human oral cavity. / Downes, Julia; Vartoukian, Sonia R.; Dewhirst, Floyd E.; Izard, Jacques; Chen, Tsute; Yu, Wen Han; Sutcliffe, Iain C.; Wade, William G.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 59, No. 5, 10.11.2009, p. 972-980.

Research output: Contribution to journalArticle

Downes, Julia ; Vartoukian, Sonia R. ; Dewhirst, Floyd E. ; Izard, Jacques ; Chen, Tsute ; Yu, Wen Han ; Sutcliffe, Iain C. ; Wade, William G. / Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum 'Synergistetes' isolated from the human oral cavity. In: International Journal of Systematic and Evolutionary Microbiology. 2009 ; Vol. 59, No. 5. pp. 972-980.
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