Purkinje cell dendrites grow in alignment with Bergmann glia

Tamar Lordkipanidze, Anna Dunaevsky

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The pattern of growth of Purkinje cell dendrites has been analyzed and related to their interactions with Bergmann glial radial processes. In cerebellar slice cultures from mice expressing green fluorescent protein (GFP) under the glial fibrillary acidic protein (GFAP) promoter, Purkinje cells were transfected and imaged with two-photon microscopy over 2 days. We report that while the Purkinje cell dendritic tree grows, individual dendrites increase or decrease in length. Importantly, we demonstrate that vertical growth of Purkinje cell dendrites occurs primarily in alignment with radial glial processes. These findings suggest that radial glial processes provide a structural substrate for the directional growth of Purkinje cell dendrites, thus influencing the shape of the dendritic tree.

Original languageEnglish (US)
Pages (from-to)229-234
Number of pages6
JournalGlia
Volume51
Issue number3
DOIs
StatePublished - Aug 15 2005

Fingerprint

Purkinje Cells
Dendrites
Neuroglia
Growth
Glial Fibrillary Acidic Protein
Green Fluorescent Proteins
Photons
Microscopy

Keywords

  • Astrocytes
  • Bergmann glia
  • Cerebellum
  • Dendrites
  • Two-photon microscopy

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Purkinje cell dendrites grow in alignment with Bergmann glia. / Lordkipanidze, Tamar; Dunaevsky, Anna.

In: Glia, Vol. 51, No. 3, 15.08.2005, p. 229-234.

Research output: Contribution to journalArticle

Lordkipanidze, Tamar ; Dunaevsky, Anna. / Purkinje cell dendrites grow in alignment with Bergmann glia. In: Glia. 2005 ; Vol. 51, No. 3. pp. 229-234.
@article{053c6bdcdc8b446bbe2c98f994a26352,
title = "Purkinje cell dendrites grow in alignment with Bergmann glia",
abstract = "The pattern of growth of Purkinje cell dendrites has been analyzed and related to their interactions with Bergmann glial radial processes. In cerebellar slice cultures from mice expressing green fluorescent protein (GFP) under the glial fibrillary acidic protein (GFAP) promoter, Purkinje cells were transfected and imaged with two-photon microscopy over 2 days. We report that while the Purkinje cell dendritic tree grows, individual dendrites increase or decrease in length. Importantly, we demonstrate that vertical growth of Purkinje cell dendrites occurs primarily in alignment with radial glial processes. These findings suggest that radial glial processes provide a structural substrate for the directional growth of Purkinje cell dendrites, thus influencing the shape of the dendritic tree.",
keywords = "Astrocytes, Bergmann glia, Cerebellum, Dendrites, Two-photon microscopy",
author = "Tamar Lordkipanidze and Anna Dunaevsky",
year = "2005",
month = "8",
day = "15",
doi = "10.1002/glia.20200",
language = "English (US)",
volume = "51",
pages = "229--234",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Purkinje cell dendrites grow in alignment with Bergmann glia

AU - Lordkipanidze, Tamar

AU - Dunaevsky, Anna

PY - 2005/8/15

Y1 - 2005/8/15

N2 - The pattern of growth of Purkinje cell dendrites has been analyzed and related to their interactions with Bergmann glial radial processes. In cerebellar slice cultures from mice expressing green fluorescent protein (GFP) under the glial fibrillary acidic protein (GFAP) promoter, Purkinje cells were transfected and imaged with two-photon microscopy over 2 days. We report that while the Purkinje cell dendritic tree grows, individual dendrites increase or decrease in length. Importantly, we demonstrate that vertical growth of Purkinje cell dendrites occurs primarily in alignment with radial glial processes. These findings suggest that radial glial processes provide a structural substrate for the directional growth of Purkinje cell dendrites, thus influencing the shape of the dendritic tree.

AB - The pattern of growth of Purkinje cell dendrites has been analyzed and related to their interactions with Bergmann glial radial processes. In cerebellar slice cultures from mice expressing green fluorescent protein (GFP) under the glial fibrillary acidic protein (GFAP) promoter, Purkinje cells were transfected and imaged with two-photon microscopy over 2 days. We report that while the Purkinje cell dendritic tree grows, individual dendrites increase or decrease in length. Importantly, we demonstrate that vertical growth of Purkinje cell dendrites occurs primarily in alignment with radial glial processes. These findings suggest that radial glial processes provide a structural substrate for the directional growth of Purkinje cell dendrites, thus influencing the shape of the dendritic tree.

KW - Astrocytes

KW - Bergmann glia

KW - Cerebellum

KW - Dendrites

KW - Two-photon microscopy

UR - http://www.scopus.com/inward/record.url?scp=23844482662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23844482662&partnerID=8YFLogxK

U2 - 10.1002/glia.20200

DO - 10.1002/glia.20200

M3 - Article

C2 - 15800897

AN - SCOPUS:23844482662

VL - 51

SP - 229

EP - 234

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 3

ER -