Purified rat lens aldose reductase. Polyol production in vitro and its inhibition by aldose reductase inhibitors

Peter F Kador, J. H. Kinoshita, D. R. Brittain, D. J. Mirrlees, C. M. Sennitt, D. Stribling

Research output: Contribution to journalArticle

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Abstract

The production of polyols in vitro by highly purified aldose reductase (EC 1.1.1.21) was monitored by g.l.c. In the presence of NADPH aldose reductase reduced glucose, galactose and xylose to the respective polyols sorbitol, galactitol and xylitol. The rates of formation of these polyols closely mirrored the K(m) values for the substrates obtained from kinetic measurements that monitored the rate of disappearance of NADPH. No polyol production occurred in the absence of purified aldose reductase, and analysis by g.l.c. revealed only the presence of unchanged monosaccharides. Addition of the aldose reductase inhibitor sorbinil to purified rat lens aldose reductase incubated with xylose in the presence of NADPH resulted in decreased xylitol production. However, aldose reductase inhibitors produced no effect in altering the rate of Nitro Blue Tetrazolium formation from either glucose or xylose, indicating that the observed inhibition in vitro does not result from a free-radical-scavenger effect.

Original languageEnglish (US)
Pages (from-to)233-237
Number of pages5
JournalBiochemical Journal
Volume240
Issue number1
DOIs
StatePublished - Jan 1 1986

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Aldehyde Reductase
Lenses
Rats
Xylose
NADP
Xylitol
Galactitol
Glucose
Free Radical Scavengers
Sorbitol
Monosaccharides
Galactose
polyol
In Vitro Techniques
Kinetics
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Purified rat lens aldose reductase. Polyol production in vitro and its inhibition by aldose reductase inhibitors. / Kador, Peter F; Kinoshita, J. H.; Brittain, D. R.; Mirrlees, D. J.; Sennitt, C. M.; Stribling, D.

In: Biochemical Journal, Vol. 240, No. 1, 01.01.1986, p. 233-237.

Research output: Contribution to journalArticle

Kador, Peter F ; Kinoshita, J. H. ; Brittain, D. R. ; Mirrlees, D. J. ; Sennitt, C. M. ; Stribling, D. / Purified rat lens aldose reductase. Polyol production in vitro and its inhibition by aldose reductase inhibitors. In: Biochemical Journal. 1986 ; Vol. 240, No. 1. pp. 233-237.
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