Purificación del factor IX recombinante expresado en leche de cerdas transgénicas

Translated title of the contribution: Purification of recombinant factor IX expressed in milk of transgenic sows

Armando Cádiz Lahens, Aniel Moya Torres, William H Velander, Manuel Limonta Vidal, Lázaro Joo Sánchez, Alexandro Rodríguez Rojas, Laritza Solarana Tito, Alina Díaz Concepción, Patricia Caunedo Almagro, Dunia Castillo González, Delfina Almagro Vázquez

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Recombinant factor IX was purified from milk of transgenic sows, and yieldings between 1 and 2 g of this protein per liter were obtained. This is a new way to get this product with a high efficiency, since its expression is 1 000 times higher than of the human plasmatic factor IX. By combining 2 chromatographic steps (ion exchange in DEAE-Shephadex A-50 and affinity chromatography with heparin), the factor IX was purified, with this milk as a starting material. Different methods were studied to eliminate caseins, the main pollutant of the process, and ultracentrifugation was selected due to its numerous advantages over the isoelectric precipitation and salt precipitation. Factor IX may be purified from transgenic milk with an elevated purity by chromatographic methods that do not use immunoaffinity and are finally scalable in industrial production, which provides new perspectives for treating hemophilia B by preparing new oral formulations.

Original languageSpanish
JournalRevista Cubana de Hematologia, Inmunologia y Hemoterapia
Volume22
Issue number2
StatePublished - May 1 2006

Fingerprint

Factor IX
Milk
Hemophilia B
Ion Exchange
Ultracentrifugation
Caseins
Affinity Chromatography
Heparin
Salts
Proteins

Keywords

  • Factor IX
  • Hemophilia B
  • Purification

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Lahens, A. C., Torres, A. M., Velander, W. H., Vidal, M. L., Sánchez, L. J., Rojas, A. R., ... Vázquez, D. A. (2006). Purificación del factor IX recombinante expresado en leche de cerdas transgénicas. Revista Cubana de Hematologia, Inmunologia y Hemoterapia, 22(2).

Purificación del factor IX recombinante expresado en leche de cerdas transgénicas. / Lahens, Armando Cádiz; Torres, Aniel Moya; Velander, William H; Vidal, Manuel Limonta; Sánchez, Lázaro Joo; Rojas, Alexandro Rodríguez; Tito, Laritza Solarana; Concepción, Alina Díaz; Almagro, Patricia Caunedo; González, Dunia Castillo; Vázquez, Delfina Almagro.

In: Revista Cubana de Hematologia, Inmunologia y Hemoterapia, Vol. 22, No. 2, 01.05.2006.

Research output: Contribution to journalArticle

Lahens, AC, Torres, AM, Velander, WH, Vidal, ML, Sánchez, LJ, Rojas, AR, Tito, LS, Concepción, AD, Almagro, PC, González, DC & Vázquez, DA 2006, 'Purificación del factor IX recombinante expresado en leche de cerdas transgénicas', Revista Cubana de Hematologia, Inmunologia y Hemoterapia, vol. 22, no. 2.
Lahens, Armando Cádiz ; Torres, Aniel Moya ; Velander, William H ; Vidal, Manuel Limonta ; Sánchez, Lázaro Joo ; Rojas, Alexandro Rodríguez ; Tito, Laritza Solarana ; Concepción, Alina Díaz ; Almagro, Patricia Caunedo ; González, Dunia Castillo ; Vázquez, Delfina Almagro. / Purificación del factor IX recombinante expresado en leche de cerdas transgénicas. In: Revista Cubana de Hematologia, Inmunologia y Hemoterapia. 2006 ; Vol. 22, No. 2.
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