Purification of poly-β-hydroxybutyrate by density gradient centrifugation in sodium bromide

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly-β-hydroxybutyrate. This technique generated high yields of membrane-bound and unbound granules of exceptional purity and degree of polymerization.

Original languageEnglish (US)
Pages (from-to)1208-1209
Number of pages2
JournalApplied and environmental microbiology
Volume43
Issue number5
StatePublished - Jan 1 1982

Fingerprint

polyhydroxybutyrate
Hydroxybutyrates
Bacillus thuringiensis
Density Gradient Centrifugation
bromide
polymerization
Polymerization
purity
purification
granules
fractionation
sodium
membrane
Membranes
methodology
sodium bromide
density gradient centrifugation
centrifugation

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Purification of poly-β-hydroxybutyrate by density gradient centrifugation in sodium bromide. / Nickerson, Kenneth.

In: Applied and environmental microbiology, Vol. 43, No. 5, 01.01.1982, p. 1208-1209.

Research output: Contribution to journalArticle

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