Purification of α-amylase from Bacillus licheniformis by chromatofocusing and gel filtration chromatography

M. Damodara Rao, A. Purnima, D. V. Ramesh, C. Ayyanna

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A combination of chromatofocusing and gel filtration chromatography resulted in a simple purification of α-amylase from Bacillus licheniformis. The purification was approximately 77-fold. Identification of the purity was established by SDS-PAGE. Molecular weight and isoelectric point of the purified enzyme were 58 kDa and 7.18 respectively. Western blot analysis confirms the specificity of antibody raised against purified α-amylase.

Original languageEnglish (US)
Pages (from-to)547-550
Number of pages4
JournalWorld Journal of Microbiology and Biotechnology
Volume18
Issue number6
DOIs
StatePublished - Aug 23 2002

Fingerprint

Bacillus licheniformis
Bacilli
Amylases
Chromatography
amylases
Purification
Gel Chromatography
Gels
Antibody Specificity
Isoelectric Point
isoelectric point
purity
Polyacrylamide Gel Electrophoresis
Western blotting
Molecular Weight
Western Blotting
Molecular weight
molecular weight
Antibodies
Enzymes

Keywords

  • Chromatofocusing
  • Gel filtration chromatography
  • α-Amylase

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Cite this

Purification of α-amylase from Bacillus licheniformis by chromatofocusing and gel filtration chromatography. / Damodara Rao, M.; Purnima, A.; Ramesh, D. V.; Ayyanna, C.

In: World Journal of Microbiology and Biotechnology, Vol. 18, No. 6, 23.08.2002, p. 547-550.

Research output: Contribution to journalArticle

@article{5d9aa8b48a82459fb0b8075c17b6b0af,
title = "Purification of α-amylase from Bacillus licheniformis by chromatofocusing and gel filtration chromatography",
abstract = "A combination of chromatofocusing and gel filtration chromatography resulted in a simple purification of α-amylase from Bacillus licheniformis. The purification was approximately 77-fold. Identification of the purity was established by SDS-PAGE. Molecular weight and isoelectric point of the purified enzyme were 58 kDa and 7.18 respectively. Western blot analysis confirms the specificity of antibody raised against purified α-amylase.",
keywords = "Chromatofocusing, Gel filtration chromatography, α-Amylase",
author = "{Damodara Rao}, M. and A. Purnima and Ramesh, {D. V.} and C. Ayyanna",
year = "2002",
month = "8",
day = "23",
doi = "10.1023/A:1016374228444",
language = "English (US)",
volume = "18",
pages = "547--550",
journal = "Mircen Journal of Applied Microbiology and Biotechnology",
issn = "0265-0762",
publisher = "Springer Netherlands",
number = "6",

}

TY - JOUR

T1 - Purification of α-amylase from Bacillus licheniformis by chromatofocusing and gel filtration chromatography

AU - Damodara Rao, M.

AU - Purnima, A.

AU - Ramesh, D. V.

AU - Ayyanna, C.

PY - 2002/8/23

Y1 - 2002/8/23

N2 - A combination of chromatofocusing and gel filtration chromatography resulted in a simple purification of α-amylase from Bacillus licheniformis. The purification was approximately 77-fold. Identification of the purity was established by SDS-PAGE. Molecular weight and isoelectric point of the purified enzyme were 58 kDa and 7.18 respectively. Western blot analysis confirms the specificity of antibody raised against purified α-amylase.

AB - A combination of chromatofocusing and gel filtration chromatography resulted in a simple purification of α-amylase from Bacillus licheniformis. The purification was approximately 77-fold. Identification of the purity was established by SDS-PAGE. Molecular weight and isoelectric point of the purified enzyme were 58 kDa and 7.18 respectively. Western blot analysis confirms the specificity of antibody raised against purified α-amylase.

KW - Chromatofocusing

KW - Gel filtration chromatography

KW - α-Amylase

UR - http://www.scopus.com/inward/record.url?scp=0036333319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036333319&partnerID=8YFLogxK

U2 - 10.1023/A:1016374228444

DO - 10.1023/A:1016374228444

M3 - Article

AN - SCOPUS:0036333319

VL - 18

SP - 547

EP - 550

JO - Mircen Journal of Applied Microbiology and Biotechnology

JF - Mircen Journal of Applied Microbiology and Biotechnology

SN - 0265-0762

IS - 6

ER -