Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana

Mamta Bajaj, Hideaki Moriyama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 Å resolution using Cu Kα radiation. The crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 69.90, b = 70.86 Å, c = 75.55 Å. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a VM of 1.8 Å3 Da-1.

Original languageEnglish (US)
Article numberpu5183
Pages (from-to)409-411
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume63
Issue number5
DOIs
StatePublished - Apr 28 2007

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Crystallization
Arabidopsis
purification
genes
Purification
Genes
crystallization
ammonium sulfates
Ammonium Sulfate
trimers
X-Ray Diffraction
Vapors
vapors
Radiation
X ray diffraction
Crystals
radiation
products
cells
diffraction

Keywords

  • Arabidopsis thaliana
  • Deoxyuridine triphosphate nucleotidohydrolase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

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abstract = "The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 {\AA} resolution using Cu Kα radiation. The crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 69.90, b = 70.86 {\AA}, c = 75.55 {\AA}. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30{\%}, with a VM of 1.8 {\AA}3 Da-1.",
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