Purification and primary structure of galanin from the alligator stomach

Yunxia Wang, J. Michael Conlon

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Galanin-like immunoreactivity (6 pmol/g tissue) was detected by radioimmunoassay in an extract of the stomach of the alligator, Alligator mississipiensis, but the peptide was present only in low concentration (<0.5 pmol/g) in extracts of the brain and small intestine. Alligator galanin comprises 29 amino acid residues and contains an α-amidated C-terminal residue. Residues 1-22 of alligator galanin are identical to the corresponding sequence in pig/sheep/rat galanins, demonstrating that strong evolutionary pressure has acted to conserve the receptor-binding domain of the peptide. Unexpectedly, in view of the close phylogenetic relationship between crocodilians and birds, alligator galanin is structurally more similar to sheep galanin (three amino acid substitutions) than to chicken galanin (four amino acid substitutions).

Original languageEnglish (US)
Pages (from-to)603-606
Number of pages4
JournalPeptides
Volume15
Issue number4
DOIs
StatePublished - 1994

Fingerprint

Alligators and Crocodiles
Galanin
Purification
Stomach
Amino Acid Substitution
Amino Acids
Sheep
Substitution reactions
Peptides
Birds
Small Intestine
Radioimmunoassay
Rats
Chickens
Brain
Swine
Tissue
Pressure

Keywords

  • Alligator
  • Chicken
  • Galanin
  • Reptile
  • Stomach

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Purification and primary structure of galanin from the alligator stomach. / Wang, Yunxia; Conlon, J. Michael.

In: Peptides, Vol. 15, No. 4, 1994, p. 603-606.

Research output: Contribution to journalArticle

Wang, Yunxia ; Conlon, J. Michael. / Purification and primary structure of galanin from the alligator stomach. In: Peptides. 1994 ; Vol. 15, No. 4. pp. 603-606.
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