Purification and characterization of a DNA-binding protein activated by ionizing radiation

B. Teale, S. Singh, Kum Khanna Kum Kum Khanna, D. Findik, M. F. Lavin

Research output: Contribution to journalArticle

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Abstract

Exposure of mammalian cells to a variety of agents leads to the activation of pre-existing proteins and the induction of specific genes. We have recently described the appearance of a specific DNA-binding protein in nuclei from cells exposed to ionizing radiation (Singh, S. P., and Lavin, M. F. (1990) Mol. Cell. Biol. 10, 5279-5285). This protein is present in the cytoplasm of unperturbed cells and is apparently translocated to the nucleus in response to radiation damage. We describe here the purification and characterization of this specific DNA-binding protein. Purification involved the use of affinity chromatography employing a multimeric form of the DNA- binding motif conjugated to cyanogen bromide-activated Sepharose. Three DNA- binding species were recognized by UV-cross-linking and South-Western analysis. The major species or that with the highest affinity was approximately 70 kDa in size. DNase-1 footprint analysis revealed a single binding site in the κ immunoglobulin gene enhancer and in a putative control sequence upstream from the c-myc gene. At salt concentrations as high as 1 M, up to 40% of the DNA-binding activity was maintained and the K(d) was calculated to be 1.205 x 10-6 M-1. Binding activity was found to be modulated by phosphorylation. Removal of phosphate groups from the protein resulted in a major loss of binding activity. It is not clear at this stage whether the factor(s) described here plays a role in transcription control or a more general DNA-processing role in response to radiation damage.

Original languageEnglish (US)
Pages (from-to)10295-10301
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number15
StatePublished - Jan 1 1992

Fingerprint

Ionizing radiation
DNA-Binding Proteins
Ionizing Radiation
Purification
Genes
DNA
Radiation damage
Radiation
Cyanogen Bromide
Immunoglobulin Genes
Nucleotide Motifs
Proteins
myc Genes
Deoxyribonucleases
Cell Nucleus
Affinity Chromatography
Affinity chromatography
Sepharose
Phosphorylation
Cytoplasm

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Teale, B., Singh, S., Kum Kum Khanna, K. K., Findik, D., & Lavin, M. F. (1992). Purification and characterization of a DNA-binding protein activated by ionizing radiation. Journal of Biological Chemistry, 267(15), 10295-10301.

Purification and characterization of a DNA-binding protein activated by ionizing radiation. / Teale, B.; Singh, S.; Kum Kum Khanna, Kum Khanna; Findik, D.; Lavin, M. F.

In: Journal of Biological Chemistry, Vol. 267, No. 15, 01.01.1992, p. 10295-10301.

Research output: Contribution to journalArticle

Teale, B, Singh, S, Kum Kum Khanna, KK, Findik, D & Lavin, MF 1992, 'Purification and characterization of a DNA-binding protein activated by ionizing radiation', Journal of Biological Chemistry, vol. 267, no. 15, pp. 10295-10301.
Teale B, Singh S, Kum Kum Khanna KK, Findik D, Lavin MF. Purification and characterization of a DNA-binding protein activated by ionizing radiation. Journal of Biological Chemistry. 1992 Jan 1;267(15):10295-10301.
Teale, B. ; Singh, S. ; Kum Kum Khanna, Kum Khanna ; Findik, D. ; Lavin, M. F. / Purification and characterization of a DNA-binding protein activated by ionizing radiation. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 15. pp. 10295-10301.
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