PTEN controls immunoreceptor (immunoreceptor tyrosine-based activation motif) signaling and the activation of Rac

Jong Suk Kim, Xiaodong Peng, Pradip K. De, Robert L. Geahlen, Donald L. Durden

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Fcγ receptor-mediated phagocytosis is a model for the study of immunoreceptor (immunoreceptor tyrosine-based activation motif [ITAM]) signaling and involves the activation of protein tyrosine kinases, protein tyrosine phosphatases, and downstream effectors including phosphatidylinositol-3 (PI-3) kinase. Relatively little is known of the role of lipid phosphatases in the control of ITAM signaling and inflammation. A heterologous COS7 cell system was used to examine the roles played by PI-3 kinase and the dual-specificity phosphatase, phosphatase and tensin homolog deleted on chromosome 10 (PTEN), in the signal transduction pathway leading to Fcγ receptor IIA-mediated phagocytosis and the activation of Rac. The expression of wildtype PTEN completely abrogated the phagocytosis of immunoglobulin-G-sensitized sheep red blood cells, as compared with the catalytically inactive mutant of PTEN, which had no effect. This is the first direct evidence that PTEN, an inositol 3′ phosphatase, regulates Fcγ receptor-mediated phagocytosis, an ITAM-based signaling event. The data suggest that PTEN exerts control over phagocytosis potentially by controlling the downstream conversion of guanosine diphosphate-Rac to guanosine triphosphate-Rac following ITAM stimulation.

Original languageEnglish (US)
Pages (from-to)694-697
Number of pages4
JournalBlood
Volume99
Issue number2
DOIs
StatePublished - Jan 15 2002

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Immunoreceptor Tyrosine-Based Activation Motif
Phagocytosis
Tyrosine
Chemical activation
Fc Receptors
Phosphatidylinositol 3-Kinase
Phosphoric Monoester Hydrolases
Dual-Specificity Phosphatases
Chromosomes, Human, Pair 10
Protein Tyrosine Phosphatases
Guanosine
Diphosphates
Signal transduction
Guanosine Triphosphate
Protein-Tyrosine Kinases
Inositol
Chromosomes
Signal Transduction
Sheep
Immunoglobulin G

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

PTEN controls immunoreceptor (immunoreceptor tyrosine-based activation motif) signaling and the activation of Rac. / Kim, Jong Suk; Peng, Xiaodong; De, Pradip K.; Geahlen, Robert L.; Durden, Donald L.

In: Blood, Vol. 99, No. 2, 15.01.2002, p. 694-697.

Research output: Contribution to journalArticle

Kim, Jong Suk ; Peng, Xiaodong ; De, Pradip K. ; Geahlen, Robert L. ; Durden, Donald L. / PTEN controls immunoreceptor (immunoreceptor tyrosine-based activation motif) signaling and the activation of Rac. In: Blood. 2002 ; Vol. 99, No. 2. pp. 694-697.
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