Pseudomonas syringae pv. syringae Uses Proteasome Inhibitor Syringolin A to Colonize from Wound Infection Sites

Johana C. Misas-Villamil, Izabella Kolodziejek, Emerson Crabill, Farnusch Kaschani, Sherry Niessen, Takayuki Shindo, Markus Kaiser, James R Alfano, Renier A.L. van der Hoorn

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Infection of plants by bacterial leaf pathogens at wound sites is common in nature. Plants defend wound sites to prevent pathogen invasion, but several pathogens can overcome spatial restriction and enter leaf tissues. The molecular mechanisms used by pathogens to suppress containment at wound infection sites are poorly understood. Here, we studied Pseudomonas syringae strains causing brown spot on bean and blossom blight on pear. These strains exist as epiphytes that can cause disease upon wounding caused by hail, sand storms and frost. We demonstrate that these strains overcome spatial restriction at wound sites by producing syringolin A (SylA), a small molecule proteasome inhibitor. Consequently, SylA-producing strains are able to escape from primary infection sites and colonize adjacent tissues along the vasculature. We found that SylA diffuses from the primary infection site and suppresses acquired resistance in adjacent tissues by blocking signaling by the stress hormone salicylic acid (SA). Thus, SylA diffusion creates a zone of SA-insensitive tissue that is prepared for subsequent colonization. In addition, SylA promotes bacterial motility and suppresses immune responses at the primary infection site. These local immune responses do not affect bacterial growth and were weak compared to effector-triggered immunity. Thus, SylA facilitates colonization from wounding sites by increasing bacterial motility and suppressing SA signaling in adjacent tissues.

Original languageEnglish (US)
Article numbere1003281
JournalPLoS pathogens
Volume9
Issue number3
DOIs
StatePublished - Apr 15 2013

Fingerprint

Pseudomonas syringae
Proteasome Inhibitors
Wound Infection
Salicylic Acid
Wounds and Injuries
Infection
Pyrus
Plant Leaves
Bacterial Infections
syringolin A
Immunity
Hormones
Growth

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Misas-Villamil, J. C., Kolodziejek, I., Crabill, E., Kaschani, F., Niessen, S., Shindo, T., ... van der Hoorn, R. A. L. (2013). Pseudomonas syringae pv. syringae Uses Proteasome Inhibitor Syringolin A to Colonize from Wound Infection Sites. PLoS pathogens, 9(3), [e1003281]. https://doi.org/10.1371/journal.ppat.1003281

Pseudomonas syringae pv. syringae Uses Proteasome Inhibitor Syringolin A to Colonize from Wound Infection Sites. / Misas-Villamil, Johana C.; Kolodziejek, Izabella; Crabill, Emerson; Kaschani, Farnusch; Niessen, Sherry; Shindo, Takayuki; Kaiser, Markus; Alfano, James R; van der Hoorn, Renier A.L.

In: PLoS pathogens, Vol. 9, No. 3, e1003281, 15.04.2013.

Research output: Contribution to journalArticle

Misas-Villamil, JC, Kolodziejek, I, Crabill, E, Kaschani, F, Niessen, S, Shindo, T, Kaiser, M, Alfano, JR & van der Hoorn, RAL 2013, 'Pseudomonas syringae pv. syringae Uses Proteasome Inhibitor Syringolin A to Colonize from Wound Infection Sites', PLoS pathogens, vol. 9, no. 3, e1003281. https://doi.org/10.1371/journal.ppat.1003281
Misas-Villamil JC, Kolodziejek I, Crabill E, Kaschani F, Niessen S, Shindo T et al. Pseudomonas syringae pv. syringae Uses Proteasome Inhibitor Syringolin A to Colonize from Wound Infection Sites. PLoS pathogens. 2013 Apr 15;9(3). e1003281. https://doi.org/10.1371/journal.ppat.1003281
Misas-Villamil, Johana C. ; Kolodziejek, Izabella ; Crabill, Emerson ; Kaschani, Farnusch ; Niessen, Sherry ; Shindo, Takayuki ; Kaiser, Markus ; Alfano, James R ; van der Hoorn, Renier A.L. / Pseudomonas syringae pv. syringae Uses Proteasome Inhibitor Syringolin A to Colonize from Wound Infection Sites. In: PLoS pathogens. 2013 ; Vol. 9, No. 3.
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