Pseudomonas syringae catalases are collectively required for plant pathogenesis

Ming Guo, Anna Block, Crystal D. Bryan, Donald F Becker, James R Alfano

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The bacterial pathogen Pseudomonas syringae pv. tomato DC3000 must detoxify plant-produced hydrogen peroxide (H 2 O 2 ) in order to survive in its host plant. Candidate enzymes for this detoxification include the monofunctional catalases KatB and KatE and the bifunctional catalase-peroxidase KatG of DC3000. This study shows that KatG is the major housekeeping catalase of DC3000 and provides protection against menadione-generated endogenous H 2 O 2 . In contrast, KatB rapidly and substantially accumulates in response to exogenous H 2 O 2 . Furthermore, KatB and KatG have nonredundant roles in detoxifying exogenous H2O2 and are required for full virulence of DC3000 in Arabidopsis thaliana. Therefore, the nonredundant ability of KatB and KatG to detoxify plant-produced H 2 O 2 is essential for the bacteria to survive in plants. Indeed, a DC3000 catalase triple mutant is severely compromised in its ability to grow in planta, and its growth can be partially rescued by the expression of katB, katE, or katG. Interestingly, our data demonstrate that although KatB and KatG are the major catalases involved in the virulence of DC3000, KatE can also provide some protection in planta. Thus, our results indicate that these catalases are virulence factors for DC3000 and are collectively required for pathogenesis.

Original languageEnglish (US)
Pages (from-to)5054-5064
Number of pages11
JournalJournal of bacteriology
Volume194
Issue number18
DOIs
StatePublished - Sep 1 2012

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Pseudomonas syringae
Catalase
Virulence
Vitamin K 3
Housekeeping
Virulence Factors
Lycopersicon esculentum
Arabidopsis
Hydrogen Peroxide
Peroxidase
Bacteria
Enzymes
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Pseudomonas syringae catalases are collectively required for plant pathogenesis. / Guo, Ming; Block, Anna; Bryan, Crystal D.; Becker, Donald F; Alfano, James R.

In: Journal of bacteriology, Vol. 194, No. 18, 01.09.2012, p. 5054-5064.

Research output: Contribution to journalArticle

Guo, Ming ; Block, Anna ; Bryan, Crystal D. ; Becker, Donald F ; Alfano, James R. / Pseudomonas syringae catalases are collectively required for plant pathogenesis. In: Journal of bacteriology. 2012 ; Vol. 194, No. 18. pp. 5054-5064.
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