Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7

Valerie Nicaise, Anna Joe, Byeong Ryool Jeong, Christin Korneli, Freddy Boutrot, Isa Westedt, Dorothee Staiger, James R. Alfano, Cyril Zipfel

Research output: Contribution to journalReview article

75 Citations (Scopus)

Abstract

Pathogens target important components of host immunity to cause disease. The Pseudomonas syringae type III-secreted effector HopU1 is a mono-ADP-ribosyltransferase required for full virulence on Arabidopsis thaliana. HopU1 targets several RNA-binding proteins including GRP7, whose role in immunity is still unclear. Here, we show that GRP7 associates with translational components, as well as with the pattern recognition receptors FLS2 and EFR. Moreover, GRP7 binds specifically FLS2 and EFR transcripts in vivo through its RNA recognition motif. HopU1 does not affect the protein-protein associations between GRP7, FLS2 and translational components. Instead, HopU1 blocks the interaction between GRP7 and FLS2 and EFR transcripts in vivo. This inhibition correlates with reduced FLS2 protein levels upon Pseudomonas infection in a HopU1-dependent manner. Our results reveal a novel virulence strategy used by a microbial effector to interfere with host immunity.

Original languageEnglish (US)
Pages (from-to)701-712
Number of pages12
JournalEMBO Journal
Volume32
Issue number5
DOIs
StatePublished - Mar 6 2013

Fingerprint

Pseudomonas
Immunity
Messenger RNA
Virulence
ADP Ribose Transferases
Pseudomonas syringae
Pattern Recognition Receptors
Pseudomonas Infections
Proteins
RNA-Binding Proteins
Pathogens
Arabidopsis
RNA

Keywords

  • RNA-binding protein
  • bacteria
  • innate immunity
  • mono-ADP-ribosyltransferase
  • pattern-recognition receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Nicaise, V., Joe, A., Jeong, B. R., Korneli, C., Boutrot, F., Westedt, I., ... Zipfel, C. (2013). Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7. EMBO Journal, 32(5), 701-712. https://doi.org/10.1038/emboj.2013.15

Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7. / Nicaise, Valerie; Joe, Anna; Jeong, Byeong Ryool; Korneli, Christin; Boutrot, Freddy; Westedt, Isa; Staiger, Dorothee; Alfano, James R.; Zipfel, Cyril.

In: EMBO Journal, Vol. 32, No. 5, 06.03.2013, p. 701-712.

Research output: Contribution to journalReview article

Nicaise, V, Joe, A, Jeong, BR, Korneli, C, Boutrot, F, Westedt, I, Staiger, D, Alfano, JR & Zipfel, C 2013, 'Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7', EMBO Journal, vol. 32, no. 5, pp. 701-712. https://doi.org/10.1038/emboj.2013.15
Nicaise, Valerie ; Joe, Anna ; Jeong, Byeong Ryool ; Korneli, Christin ; Boutrot, Freddy ; Westedt, Isa ; Staiger, Dorothee ; Alfano, James R. ; Zipfel, Cyril. / Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7. In: EMBO Journal. 2013 ; Vol. 32, No. 5. pp. 701-712.
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