Pseudomonas fluorescens JH 70-4 promotes Pb stabilization and early seedling growth of Sudan grass in contaminated mining site soil

Jaehong Shim, A. Giridhar Babu, Palanivel Velmurugan, Patrick J. Shea, Byung Taek Oh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A bacterial strain (JH 70-4) exhibiting plant growth promoting characteristics (indoleacetic acid production and 1-aminocyclopropane-1- carboxylate deaminase activity), as well as heavy metal(loid) (HM) tolerance and Pb precipitation, was isolated from HM-contaminated soil at an abandoned mine site. The bacterium was identified as Pseudomonas fluorescens based on 16S rDNA sequencing. The JH 70-4 strain induced precipitation of Pb as PbS nanoparticles, confirmed by X-ray diffraction. Solution pH, incubation time, and Pb concentration influenced removal and PbS formation. Inoculating contaminated soil with JH 70-4 decreased Pb availability; exchangeable Pb decreased while organic- and sulphide-bound Pb increased. The toxicity characteristic leaching procedure showed a 65% decrease in Pb in leachate 60 d after inoculating soil with JH 70-4. Shoot and root lengths of Sudan grass grown in the inoculated soil were greater than in the uninoculated soil. Findings suggest that microbial Pb fixation is a viable strategy for remediating soil and promoting plant growth for phytostabilization of contaminated sites.

Original languageEnglish (US)
Pages (from-to)2589-2596
Number of pages8
JournalEnvironmental Technology (United Kingdom)
Volume35
Issue number20
DOIs
StatePublished - Oct 18 2014

Fingerprint

stabilization
Stabilization
seedling
grass
Soils
1-aminocyclopropane-1-carboxylate deaminase
soil
abandoned mine
Abandoned mines
fixation
leachate
shoot
tolerance
incubation
X-ray diffraction
leaching
sulfide
Sulfides
heavy metal
Heavy Metals

Keywords

  • Nanomaterials
  • Pseudomonas
  • lead sulphide
  • metal tolerance
  • plant growth promoting bacteria

ASJC Scopus subject areas

  • Environmental Chemistry
  • Water Science and Technology
  • Waste Management and Disposal

Cite this

Pseudomonas fluorescens JH 70-4 promotes Pb stabilization and early seedling growth of Sudan grass in contaminated mining site soil. / Shim, Jaehong; Babu, A. Giridhar; Velmurugan, Palanivel; Shea, Patrick J.; Oh, Byung Taek.

In: Environmental Technology (United Kingdom), Vol. 35, No. 20, 18.10.2014, p. 2589-2596.

Research output: Contribution to journalArticle

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