Proton-Translocating Adenosine Triphosphatase Activity in Lactic Acid Bacterial

Nancy L. Nannen, Robert W Hutkins

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Streptococcus thermophilus 19258 and 573, Lactococcus lactis ssp. lactis C2, Lactococcus lactis ssp. cremoris HP, and Lactobacillus casei 685 were grown in various media and assayed for proton-translocating ATPase (H+-ATPase) activity. The H+-ATPase was extracted from the membrane fractions of protoplasted cells using a French pressure cell, and activity was measured by the release of inorganic phosphate from ATP. The pH optima for the H+-ATPase, assayed in vitro, were 7.5 for S. thermophilus and Lactococcus lactis ssp. cremoris HP and 5.0 for Lactobacillus casei 685. In contrast, for cells grown in batch culture the H+-ATPase activity was always greatest when the cytoplasmic pH was less than the optimum for the enzyme. The H+-ATPase activity generally increased as the pH decreased until an extracellular pH of 5.0 was reached. Below an extracellular pH of 5.0, activities of this enzyme dropped markedly. The aciduric lactic acid bacterium, Lactobacillus casei 685, had higher basal levels of this enzyme than the streptococci and lactococci. Results suggest that this enzyme may be involved in regulation of the intracellular pH in lactic acid bacteria.

Original languageEnglish (US)
Pages (from-to)747-751
Number of pages5
JournalJournal of Dairy Science
Volume74
Issue number3
DOIs
StatePublished - Jan 1 1991

Fingerprint

Proton-Translocating ATPases
H-transporting ATP synthase
lactic acid
protons
adenosinetriphosphatase
Adenosine Triphosphatases
Protons
Lactic Acid
Lactobacillus casei
Lactococcus lactis
Streptococcus thermophilus
Enzymes
lactic acid bacteria
enzymes
Lactococcus
Bacteria
Batch Cell Culture Techniques
Streptococcus
cells
Adenosine Triphosphate

Keywords

  • ATPase
  • P
  • Streptococcus thermophilus
  • extracellular pH
  • inorganic phosphate
  • intracellular pH
  • lactic acid bacteria
  • pH
  • pH
  • pH gradient (in-tracellular pH minus extracellular pH)
  • ΔpH

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Proton-Translocating Adenosine Triphosphatase Activity in Lactic Acid Bacterial. / Nannen, Nancy L.; Hutkins, Robert W.

In: Journal of Dairy Science, Vol. 74, No. 3, 01.01.1991, p. 747-751.

Research output: Contribution to journalArticle

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