Proton inhibition of transient outward potassium current in rat ventricular myocytes

Zhi Xu, George J Rozanski

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Acidosis elicited during myocardial ischemia is a significant pathophysiological condition markedly affecting the electrical and contractile properties of heart muscle. We examined the effects of protons on K+ channel activity in rat ventricular myocytes by recording transient outward (I(to)) and inward rectifier (I(K1)) K+ currents using the whole cell, voltage clamp technique. Proton concentration was controlled by independently varying the pH of HEPES-buffered external (pH(o)) or pipette (pH(p)) solutions. Mean I(to) density in myocytes preconditioned in acidic external solution (pH(o), 6.0) for 15-20 min was significantly less than control cells equilibrated at physiological pH(o). In contrast, I(K1) was not changed during this period of acidosis. External acidification did not decrease I(to) when initiated after intracellular dialysis with standard pH(p) 7.2. However, when myocytes were dialyzed with acidic pH(p), I(to) density was significantly less than control, while alkaline pH(p) had little effect. Despite marked reduction in current density produced by low pH(p) solutions, steady-state activation and inactivation parameters of I(to) were not significantly altered. In addition, the reversal potential of this current, kinetics of inactivation and recovery from inactivation were not significantly affected by acidic or alkaline pH(p) solutions. Acidic pH(p) alone did not change I(K1) density compared with control, but when combined with Na+/H+ exchange blockade with 5-(N,N-dimethyl)-amiloride or Na+-free external solution, I(K1) density was significantly reduced. Our data suggest that protons inhibit I(to) predominantly from the intracellular side of the channel, possibly by altering its conductance or gating properties. Moreover, intracellular protons differentially affect I(to) and I(K1) channels, with the former exhibiting greater sensitivity for a given level of acidosis.

Original languageEnglish (US)
Pages (from-to)481-490
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume29
Issue number2
DOIs
StatePublished - Jan 1 1997

Fingerprint

Muscle Cells
Protons
Potassium
Acidosis
HEPES
Patch-Clamp Techniques
Myocardial Ischemia
Dialysis
Myocardium

Keywords

  • Acidosis
  • K currents
  • Rat
  • Ventricular myocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Proton inhibition of transient outward potassium current in rat ventricular myocytes. / Xu, Zhi; Rozanski, George J.

In: Journal of Molecular and Cellular Cardiology, Vol. 29, No. 2, 01.01.1997, p. 481-490.

Research output: Contribution to journalArticle

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AB - Acidosis elicited during myocardial ischemia is a significant pathophysiological condition markedly affecting the electrical and contractile properties of heart muscle. We examined the effects of protons on K+ channel activity in rat ventricular myocytes by recording transient outward (I(to)) and inward rectifier (I(K1)) K+ currents using the whole cell, voltage clamp technique. Proton concentration was controlled by independently varying the pH of HEPES-buffered external (pH(o)) or pipette (pH(p)) solutions. Mean I(to) density in myocytes preconditioned in acidic external solution (pH(o), 6.0) for 15-20 min was significantly less than control cells equilibrated at physiological pH(o). In contrast, I(K1) was not changed during this period of acidosis. External acidification did not decrease I(to) when initiated after intracellular dialysis with standard pH(p) 7.2. However, when myocytes were dialyzed with acidic pH(p), I(to) density was significantly less than control, while alkaline pH(p) had little effect. Despite marked reduction in current density produced by low pH(p) solutions, steady-state activation and inactivation parameters of I(to) were not significantly altered. In addition, the reversal potential of this current, kinetics of inactivation and recovery from inactivation were not significantly affected by acidic or alkaline pH(p) solutions. Acidic pH(p) alone did not change I(K1) density compared with control, but when combined with Na+/H+ exchange blockade with 5-(N,N-dimethyl)-amiloride or Na+-free external solution, I(K1) density was significantly reduced. Our data suggest that protons inhibit I(to) predominantly from the intracellular side of the channel, possibly by altering its conductance or gating properties. Moreover, intracellular protons differentially affect I(to) and I(K1) channels, with the former exhibiting greater sensitivity for a given level of acidosis.

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