Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats

Kusum Kharbanda, Vasanthy Vigneswara, Benita L McVicker, Anna U. Newlaczyl, Kevin Bailey, Dean Tuma, David E. Ray, Wayne G. Carter

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We employed a proteomic profiling strategy to examine the effects of ethanol and betaine diet supplementation on major liver protein level changes. Male Wistar rats were fed control, ethanol or betaine supplemented diets for 4 weeks. Livers were removed and liver cytosolic proteins resolved by one-dimensional and two-dimensional separation techniques. Significant upregulation of betaine homocysteine methyltransferase-1, methionine adenosyl transferase-1, and glycine N-methyltransferase were the most visually prominent protein changes observed in livers of rats fed the betaine supplemented ethanol diet. We hypothesise that this concerted upregulation of these methionine metabolic pathway enzymes is the protective mechanism by which betaine restores a normal metabolic ratio of liver S-adenosylmethionine to S-adenosylhomocysteine. Ethanol also induced significant downregulation of carbonic anhydrase-III protein levels which was not restored by betaine supplementation. Carbonic anhydrase-III can function to resist oxidative stress, and we therefore hypothesise that carbonic anhydrase-III protein levels compromised by ethanol consumption, contribute to ethanol-induced redox stress.

Original languageEnglish (US)
Pages (from-to)523-527
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume381
Issue number4
DOIs
StatePublished - Apr 17 2009

Fingerprint

Carbonic Anhydrase III
Betaine
Metabolic Networks and Pathways
Methionine
Proteomics
Rats
Ethanol
Up-Regulation
Liver
Down-Regulation
Enzymes
Nutrition
Diet
Proteins
Glycine N-Methyltransferase
Betaine-Homocysteine S-Methyltransferase
S-Adenosylhomocysteine
S-Adenosylmethionine
Oxidative stress
Transferases

Keywords

  • Alcohol
  • Betaine
  • Carbonic anhydrase-III
  • Isoaspartate
  • Liver
  • Protein isoaspartyl methyltransferase
  • Proteomics
  • Redox stress
  • S-adenosylhomocysteine
  • S-adenosylmethionine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats. / Kharbanda, Kusum; Vigneswara, Vasanthy; McVicker, Benita L; Newlaczyl, Anna U.; Bailey, Kevin; Tuma, Dean; Ray, David E.; Carter, Wayne G.

In: Biochemical and Biophysical Research Communications, Vol. 381, No. 4, 17.04.2009, p. 523-527.

Research output: Contribution to journalArticle

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