Proteomics in aquatic amphipods

Can it be used to determine mechanisms of toxicity and interspecies responses after exposure to atrazine?

Kimberly J. Ralston-Hooper, Brian C. Sanchez, Jiri Adamec, María S. Sepúlveda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Proteomics has gained popularity in the field of ecotoxicology as a holistic tool for unraveling novel mechanisms of toxicity and elucidating subtle effects of contaminant exposure. The holoarctic amphipod Diporeia spp. is declining at precipitous rates in the Great Lakes, and we are evaluating the use of the well-studied amphipod model Hyalella azteca as a surrogate for Diporeia spp. This article presents proteomics data from both amphipod species exposed to atrazine (ATZ) and one of its metabolites, desethylatrazine (DEA; 3 and 30μg/L for 21 and 42d). We used a proteomics approach to determine whether these two species of amphipods responded similarly to the same chemicals and to understand better the mechanisms of toxicity of ATZ and DEA in aquatic invertebrates. We observed disruption in energy production and mitochondrial function as well as hormesis in exposed organisms. In addition, we identified a two proteins (GAPDH and HSP 90kDa) that have been linked to hormonal disruptions, suggesting potential endocrine disruption. Finally, we found that H. azteca and Diporeia spp. responded with similar proteomic profiles after ATZ and DEA exposure, suggesting that H. azteca may be used as a surrogate model organism for Diporeia spp.

Original languageEnglish (US)
Pages (from-to)1197-1203
Number of pages7
JournalEnvironmental Toxicology and Chemistry
Volume30
Issue number5
DOIs
StatePublished - May 1 2011

Fingerprint

Amphipoda
Atrazine
proteomics
atrazine
amphipod
Proteomics
Toxicity
toxicity
Hormesis
Ecotoxicology
ecotoxicology
Invertebrates
Lakes
Metabolites
metabolite
invertebrate
Impurities
protein
exposure
pollutant

Keywords

  • Atrazine
  • Desethylatrazine
  • Diporeia
  • Hyalella
  • Proteomics

ASJC Scopus subject areas

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis

Cite this

Proteomics in aquatic amphipods : Can it be used to determine mechanisms of toxicity and interspecies responses after exposure to atrazine? / Ralston-Hooper, Kimberly J.; Sanchez, Brian C.; Adamec, Jiri; Sepúlveda, María S.

In: Environmental Toxicology and Chemistry, Vol. 30, No. 5, 01.05.2011, p. 1197-1203.

Research output: Contribution to journalArticle

@article{24f9f1e8439d43998389143778269159,
title = "Proteomics in aquatic amphipods: Can it be used to determine mechanisms of toxicity and interspecies responses after exposure to atrazine?",
abstract = "Proteomics has gained popularity in the field of ecotoxicology as a holistic tool for unraveling novel mechanisms of toxicity and elucidating subtle effects of contaminant exposure. The holoarctic amphipod Diporeia spp. is declining at precipitous rates in the Great Lakes, and we are evaluating the use of the well-studied amphipod model Hyalella azteca as a surrogate for Diporeia spp. This article presents proteomics data from both amphipod species exposed to atrazine (ATZ) and one of its metabolites, desethylatrazine (DEA; 3 and 30μg/L for 21 and 42d). We used a proteomics approach to determine whether these two species of amphipods responded similarly to the same chemicals and to understand better the mechanisms of toxicity of ATZ and DEA in aquatic invertebrates. We observed disruption in energy production and mitochondrial function as well as hormesis in exposed organisms. In addition, we identified a two proteins (GAPDH and HSP 90kDa) that have been linked to hormonal disruptions, suggesting potential endocrine disruption. Finally, we found that H. azteca and Diporeia spp. responded with similar proteomic profiles after ATZ and DEA exposure, suggesting that H. azteca may be used as a surrogate model organism for Diporeia spp.",
keywords = "Atrazine, Desethylatrazine, Diporeia, Hyalella, Proteomics",
author = "Ralston-Hooper, {Kimberly J.} and Sanchez, {Brian C.} and Jiri Adamec and Sep{\'u}lveda, {Mar{\'i}a S.}",
year = "2011",
month = "5",
day = "1",
doi = "10.1002/etc.475",
language = "English (US)",
volume = "30",
pages = "1197--1203",
journal = "Environmental Toxicology and Chemistry",
issn = "0730-7268",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Proteomics in aquatic amphipods

T2 - Can it be used to determine mechanisms of toxicity and interspecies responses after exposure to atrazine?

AU - Ralston-Hooper, Kimberly J.

AU - Sanchez, Brian C.

AU - Adamec, Jiri

AU - Sepúlveda, María S.

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Proteomics has gained popularity in the field of ecotoxicology as a holistic tool for unraveling novel mechanisms of toxicity and elucidating subtle effects of contaminant exposure. The holoarctic amphipod Diporeia spp. is declining at precipitous rates in the Great Lakes, and we are evaluating the use of the well-studied amphipod model Hyalella azteca as a surrogate for Diporeia spp. This article presents proteomics data from both amphipod species exposed to atrazine (ATZ) and one of its metabolites, desethylatrazine (DEA; 3 and 30μg/L for 21 and 42d). We used a proteomics approach to determine whether these two species of amphipods responded similarly to the same chemicals and to understand better the mechanisms of toxicity of ATZ and DEA in aquatic invertebrates. We observed disruption in energy production and mitochondrial function as well as hormesis in exposed organisms. In addition, we identified a two proteins (GAPDH and HSP 90kDa) that have been linked to hormonal disruptions, suggesting potential endocrine disruption. Finally, we found that H. azteca and Diporeia spp. responded with similar proteomic profiles after ATZ and DEA exposure, suggesting that H. azteca may be used as a surrogate model organism for Diporeia spp.

AB - Proteomics has gained popularity in the field of ecotoxicology as a holistic tool for unraveling novel mechanisms of toxicity and elucidating subtle effects of contaminant exposure. The holoarctic amphipod Diporeia spp. is declining at precipitous rates in the Great Lakes, and we are evaluating the use of the well-studied amphipod model Hyalella azteca as a surrogate for Diporeia spp. This article presents proteomics data from both amphipod species exposed to atrazine (ATZ) and one of its metabolites, desethylatrazine (DEA; 3 and 30μg/L for 21 and 42d). We used a proteomics approach to determine whether these two species of amphipods responded similarly to the same chemicals and to understand better the mechanisms of toxicity of ATZ and DEA in aquatic invertebrates. We observed disruption in energy production and mitochondrial function as well as hormesis in exposed organisms. In addition, we identified a two proteins (GAPDH and HSP 90kDa) that have been linked to hormonal disruptions, suggesting potential endocrine disruption. Finally, we found that H. azteca and Diporeia spp. responded with similar proteomic profiles after ATZ and DEA exposure, suggesting that H. azteca may be used as a surrogate model organism for Diporeia spp.

KW - Atrazine

KW - Desethylatrazine

KW - Diporeia

KW - Hyalella

KW - Proteomics

UR - http://www.scopus.com/inward/record.url?scp=79953732368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953732368&partnerID=8YFLogxK

U2 - 10.1002/etc.475

DO - 10.1002/etc.475

M3 - Article

VL - 30

SP - 1197

EP - 1203

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 5

ER -