Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins

Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm∗B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, and proteolysis. Principal component analysis and pathway enrichment suggested that the mutants partitioned into three groups: (i) Mc, DeB30, fl2 and o2; (ii) o1; and (iii) fl1. Indicator species analysis revealed mutant-specific proteins, and highlighted ER secretory pathway components that were enriched in selected groups of mutants. The most significantly changed proteins were related to stress or defense and zein partitioning into the soluble fraction for Mc, DeB30, o1, and fl1 specifically. In silico dissection of the most significantly changed proteins revealed novel qualitative changes in lysine abundance contributing to the overall lysine increase and the nutritional rebalancing of the o2 and fl2 endosperm.

Original languageEnglish (US)
Pages (from-to)1381-1396
Number of pages16
JournalJournal of experimental botany
Volume67
Issue number5
DOIs
StatePublished - Mar 1 2016

Fingerprint

Endosperm
Proteomics
proteomics
Lysine
endosperm
Zea mays
lysine
Zein
mutants
corn
Endoplasmic Reticulum
zein
Proteins
proteins
Prolamins
Secretory Component
Biological Phenomena
endoplasmic reticulum
Secretory Pathway
Protein Folding

Keywords

  • Endosperm
  • maize
  • non-zein
  • opaque
  • proteome
  • stress

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins. / Morton, Kyla J.; Jia, Shangang; Zhang, Chi; Holding, David R.

In: Journal of experimental botany, Vol. 67, No. 5, 01.03.2016, p. 1381-1396.

Research output: Contribution to journalArticle

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