Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry

Jason DeBoer, Melinda S. Wojtkiewicz, Nicole Haverland, Yan Li, Emma Harwood, Emily Leshen, Joseph W. George, Pawel S Ciborowski, Michael Belshan

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Viral pathogenesis results from changes in host cells due to virus usurpation of the host cell and the innate cellular responses to thwart infection. We measured global changes in protein expression and localization in HIV-1 infected T-cells using subcellular fractionation and the Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS) proteomic platform. Eight biological replicates were performed in two independent experimental series. In silico merging of both experiments identified 287 proteins with altered expression (p <.05) between control and infected cells- 172 in the cytoplasm, 84 in the membrane, and 31 in nuclei. 170 of the proteins are components of the NIH HIV interaction database. Multiple Reaction Monitoring and traditional immunoblotting validated the altered expression of several factors during infection. Numerous factors were found to affect HIV infection in gain- and loss-of-expression infection assays, including the intermediate filament vimentin which was found to be required for efficient infection.

Original languageEnglish (US)
Pages (from-to)246-257
Number of pages12
JournalVirology
Volume516
DOIs
Publication statusPublished - Mar 2018

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Keywords

  • HIV-1
  • Host response to infection
  • Proteomics
  • SWATH-MS
  • Vimentin

ASJC Scopus subject areas

  • Virology

Cite this

DeBoer, J., Wojtkiewicz, M. S., Haverland, N., Li, Y., Harwood, E., Leshen, E., ... Belshan, M. (2018). Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry. Virology, 516, 246-257. https://doi.org/10.1016/j.virol.2018.01.025