Proteomic Profiling and Analytical Chemistry: The Crossroads: Second Edition

Pawel S Ciborowski, J. Silberring

Research output: Book/ReportBook

1 Citation (Scopus)

Abstract

Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics.

Original languageEnglish (US)
PublisherElsevier Inc.
Number of pages282
ISBN (Print)9780444636881
DOIs
StatePublished - Mar 22 2016

Fingerprint

Chemical analysis
Quality control
Proteomics
Statistical tests
Biomarkers
Microarrays
Electrophoresis
Fluorescent Dyes
Design of experiments
Proteins
Gels
Experiments
Acoustic waves
Peptides

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Proteomic Profiling and Analytical Chemistry : The Crossroads: Second Edition. / Ciborowski, Pawel S; Silberring, J.

Elsevier Inc., 2016. 282 p.

Research output: Book/ReportBook

@book{be4e1557f19148d089a629f1d60bdc9b,
title = "Proteomic Profiling and Analytical Chemistry: The Crossroads: Second Edition",
abstract = "Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics.",
author = "Ciborowski, {Pawel S} and J. Silberring",
year = "2016",
month = "3",
day = "22",
doi = "10.1016/C2015-0-01616-1",
language = "English (US)",
isbn = "9780444636881",
publisher = "Elsevier Inc.",

}

TY - BOOK

T1 - Proteomic Profiling and Analytical Chemistry

T2 - The Crossroads: Second Edition

AU - Ciborowski, Pawel S

AU - Silberring, J.

PY - 2016/3/22

Y1 - 2016/3/22

N2 - Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics.

AB - Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics.

UR - http://www.scopus.com/inward/record.url?scp=84969715374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969715374&partnerID=8YFLogxK

U2 - 10.1016/C2015-0-01616-1

DO - 10.1016/C2015-0-01616-1

M3 - Book

AN - SCOPUS:84969715374

SN - 9780444636881

BT - Proteomic Profiling and Analytical Chemistry

PB - Elsevier Inc.

ER -