Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations

Yoshimi Enose, Christopher J. Destache, Andrea L. Mack, James R. Anderson, Fred Ullrich, Pawel S Ciborowski, Howard Eliot Gendelman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip® assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.

Original languageEnglish (US)
Pages (from-to)161-172
Number of pages12
JournalGlia
Volume51
Issue number3
DOIs
StatePublished - Aug 15 2005

Fingerprint

Dermatoglyphics
Microglia
Proteomics
Spleen
Bone Marrow
Macrophages
Population
Protein Array Analysis
Proteins
Macrophage Colony-Stimulating Factor
Phagocytes
Tandem Mass Spectrometry
Inbred C57BL Mouse
Liquid Chromatography
Dendritic Cells
Disease Progression
Monocytes
Membrane Proteins
Lasers
Homeostasis

Keywords

  • Cellular proteins
  • Macrophages
  • Microglia
  • Proteomics

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations. / Enose, Yoshimi; Destache, Christopher J.; Mack, Andrea L.; Anderson, James R.; Ullrich, Fred; Ciborowski, Pawel S; Gendelman, Howard Eliot.

In: Glia, Vol. 51, No. 3, 15.08.2005, p. 161-172.

Research output: Contribution to journalArticle

Enose, Yoshimi ; Destache, Christopher J. ; Mack, Andrea L. ; Anderson, James R. ; Ullrich, Fred ; Ciborowski, Pawel S ; Gendelman, Howard Eliot. / Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations. In: Glia. 2005 ; Vol. 51, No. 3. pp. 161-172.
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