Proteomic fingerprinting of human immunodeficiency virus type 1-associated dementia from patient monocyte-derived macrophages: A case study

Valerie Wojna, Kim A Carlson, Xiaoguang Luo, Raúl Mayo, Loyda M. Meléndez, Edmundo Kraiselburd, Howard Eliot Gendelman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The emergence of a subset of circulating monocytes during human immunodeficiency virus type 1 (HIV-1) disease has been shown to correlate with cognitive impairment. Thus, it is hypothesized that diagnostic protein profiles may be obtained from these cells from patients with or at risk for HIV-1-associated dementia (HAD). To address this possibility, we used ProteinChip assays to define a unique monocyte-derived macrophage (MDM) protein fingerprint during HAD and whether it is affected by highly active antiretroviral therapy (HAART). The study included five Hispanic women, one with HAD, two HIV-1-infected without cognitive impairment, and two seronegative controls. All patients were matched by age and immune status. Monocytes were recovered from the peripheral blood leukocytes by Percoll gradient centrifugation and allowed to differentiate in vitro for 7 days. Cell lysates and supernatants were collected from the MDM and analyzed by surface enhanced laser desorption/ionization-time of flight ProteinChip assays. Seven unique protein peaks between 3.0 and 20.0 kDa were found in the HAD MDM sample. Each of these proteins were abrogated after HAART. Additional studies extending this one time point determination would serve to confirm the general utility of MDM protein profiling for the diagnosis and monitoring of HAD.

Original languageEnglish (US)
Pages (from-to)74-81
Number of pages8
JournalJournal of neurovirology
Volume10
Issue numberSUPPL. 1
DOIs
StatePublished - Mar 22 2004

Fingerprint

Proteomics
Dementia
HIV-1
Macrophages
Protein Array Analysis
Highly Active Antiretroviral Therapy
Monocytes
Proteins
Peptide Mapping
Virus Diseases
Centrifugation
Hispanic Americans
Lasers
Leukocytes

Keywords

  • HAD
  • Monocytes
  • Proteomic profiles
  • Unique signature

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Virology

Cite this

Proteomic fingerprinting of human immunodeficiency virus type 1-associated dementia from patient monocyte-derived macrophages : A case study. / Wojna, Valerie; Carlson, Kim A; Luo, Xiaoguang; Mayo, Raúl; Meléndez, Loyda M.; Kraiselburd, Edmundo; Gendelman, Howard Eliot.

In: Journal of neurovirology, Vol. 10, No. SUPPL. 1, 22.03.2004, p. 74-81.

Research output: Contribution to journalArticle

Wojna, Valerie ; Carlson, Kim A ; Luo, Xiaoguang ; Mayo, Raúl ; Meléndez, Loyda M. ; Kraiselburd, Edmundo ; Gendelman, Howard Eliot. / Proteomic fingerprinting of human immunodeficiency virus type 1-associated dementia from patient monocyte-derived macrophages : A case study. In: Journal of neurovirology. 2004 ; Vol. 10, No. SUPPL. 1. pp. 74-81.
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