Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction

Arvin Bansal, Qiuxia Dai, Ying Ann Chiao, Kevin W. Hakala, John Q. Zhang, Susan T. Weintraub, Merry L Lindsey

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Exercise has been shown to improve function of the left ventricle (LV) following myocardial infarction (MI). The mechanisms to explain this benefit have not been fully delineated, but may involve improved mechanics resulting in unloading effects and increased endothelial nitric oxide synthase levels [1,2]. Accordingly, the goal of this study was to determine how the LV infarct proteome is altered by a post-MI exercise regimen. Sprague-Dawley rats underwent ligation of the left descending coronary artery to induce MI. Exercise training was initiated four weeks post-MI and continued for 8. weeks in n= 12 rats. Compared with the sedentary MI group (n= 10), the infarct region of rats receiving exercise showed 20 protein spots with altered intensities in two-dimensional gels (15 increased and 5 decreased; p< 0.05). Of 52 proteins identified in 20 spots, decreased levels of voltage-dependent anion-selective channel 2 and increased levels of glutathione perioxidase and manganese superoxide were confirmed by immunoblotting. Cardiac function was preserved in rats receiving exercise training, and the beneficial effect was linked with changes in these 3 proteins. In conclusion, our results suggest that post-MI exercise training increases anti-oxidant levels and decreases ion channel levels, which may explain, in part, the improved cardiac function seen with exercise.

Original languageEnglish (US)
Pages (from-to)2041-2049
Number of pages9
JournalJournal of Proteomics
Volume73
Issue number10
DOIs
StatePublished - Sep 1 2010

Fingerprint

Proteomics
Rats
Myocardial Infarction
Voltage-Dependent Anion Channels
Exercise
Voltage-Dependent Anion Channel 2
Proteins
Heart Ventricles
Nitric Oxide Synthase Type III
Proteome
Manganese
Unloading
Ion Channels
Oxidants
Superoxides
Glutathione
Mechanics
Gels
Immunoblotting
Ligation

Keywords

  • Anti-oxidant
  • Exercise
  • Left ventricular remodeling
  • Myocardial infarction
  • Proteomics
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction. / Bansal, Arvin; Dai, Qiuxia; Chiao, Ying Ann; Hakala, Kevin W.; Zhang, John Q.; Weintraub, Susan T.; Lindsey, Merry L.

In: Journal of Proteomics, Vol. 73, No. 10, 01.09.2010, p. 2041-2049.

Research output: Contribution to journalArticle

Bansal, Arvin ; Dai, Qiuxia ; Chiao, Ying Ann ; Hakala, Kevin W. ; Zhang, John Q. ; Weintraub, Susan T. ; Lindsey, Merry L. / Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction. In: Journal of Proteomics. 2010 ; Vol. 73, No. 10. pp. 2041-2049.
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