Proteomic analysis of six- and twelve-month hippocampus and cerebellum in a murine Down syndrome model

Guido N. Vacano, David S. Gibson, Abdullah Arif Turjoman, Jeremy W. Gawryluk, Jonathan Geiger, Mark Duncan, David Patterson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study was designed to investigate the brain proteome of the Ts65Dn mouse model of Down syndrome. We profiled the cerebellum and hippocampus proteomes of 6- and 12-month-old trisomic and disomic mice by difference gel electrophoresis. We quantified levels of 2082 protein spots and identified 272 (170 unique UniProt accessions) by mass spectrometry. Four identified proteins are encoded by genes trisomic in the Ts65Dn mouse. Three of these (CRYZL11, EZR, and SOD1) were elevated with p-value <0.05, and 2 proteins encoded by disomic genes (MAPRE3 and PHB) were reduced. Intergel comparisons based on age (6 vs. 12 months) and brain region (cerebellum vs. hippocampus) revealed numerous differences. Specifically, 132 identified proteins were different between age groups, and 141 identified proteins were different between the 2 brain regions. Our results suggest that compensatory mechanisms exist, which ameliorate the effect of trisomy in the Ts65Dn mice. Differences observed during aging may play a role in the accelerated deterioration of learning and memory seen in Ts65Dn mice.

Original languageEnglish (US)
Pages (from-to)96-109
Number of pages14
JournalNeurobiology of Aging
Volume63
DOIs
StatePublished - Mar 1 2018
Externally publishedYes

Fingerprint

Down Syndrome
Proteomics
Cerebellum
Hippocampus
Proteome
Proteins
Brain
Trisomy
Electrophoresis
Mass Spectrometry
Age Groups
Gels
Learning
Genes

Keywords

  • Aging
  • Alzheimer's disease
  • Brain
  • Down syndrome
  • Proteome
  • Ts65Dn

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

Cite this

Proteomic analysis of six- and twelve-month hippocampus and cerebellum in a murine Down syndrome model. / Vacano, Guido N.; Gibson, David S.; Turjoman, Abdullah Arif; Gawryluk, Jeremy W.; Geiger, Jonathan; Duncan, Mark; Patterson, David.

In: Neurobiology of Aging, Vol. 63, 01.03.2018, p. 96-109.

Research output: Contribution to journalArticle

Vacano, Guido N. ; Gibson, David S. ; Turjoman, Abdullah Arif ; Gawryluk, Jeremy W. ; Geiger, Jonathan ; Duncan, Mark ; Patterson, David. / Proteomic analysis of six- and twelve-month hippocampus and cerebellum in a murine Down syndrome model. In: Neurobiology of Aging. 2018 ; Vol. 63. pp. 96-109.
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