Proteomic analysis of neuronal mitochondria

Kelly L. Stauch, Howard S Fox

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Neurons critically depend on mitochondrial function to establish membrane excitability and to execute the complex processes of neurotransmission and plasticity. Although mitochondria are central for various cellular processes important for neuronal function that include ATP production, intracellular calcium signaling, and generation of reactive oxygen species, our understanding of the roles of mitochondria in neurons and intact brain tissue are limited. Proteins carry out an array of mitochondrial functions, with structural, transporter, hormone, enzyme, and receptor protein classes working in complexes and pathways to serve these critical functions. Knowledge of the protein composition of mitochondria and how it changes under different conditions will yield important information to further our insight into mitochondrial processes. Here we describe the implementation of mass spectrometry (MS)-based proteomics to the study of neuronal mitochondria, with emphasis on quantitative approaches. This chapter describes the isolation of mitochondria from primary neuronal cultures and brain tissue nerve terminals, sample preparation for liquid chromatography dual MS (LC-MS/MS), the LC-MS/MS procedure, and the subsequent data processing steps to compare global mitochondrial proteomic alterations. Identifying mitochondrial proteins that are altered within neurons during development and disease is of central importance for understanding the multifaceted roles of mitochondria.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages299-319
Number of pages21
DOIs
StatePublished - Jan 1 2017

Publication series

NameNeuromethods
Volume123
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Fingerprint

Mitochondria
Proteomics
Mass spectrometry
Neurons
Tandem Mass Spectrometry
Brain
Mass Spectrometry
Tissue
Nerve Tissue
Proteins
Calcium Signaling
Mitochondrial Proteins
Liquid chromatography
Cell culture
Synaptic Transmission
Liquid Chromatography
Plasticity
Reactive Oxygen Species
Adenosine Triphosphate
Hormones

Keywords

  • Mass spectrometry
  • Mitochondria
  • Neurons
  • Proteomics
  • Synaptosomes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Cite this

Stauch, K. L., & Fox, H. S. (2017). Proteomic analysis of neuronal mitochondria. In Neuromethods (pp. 299-319). (Neuromethods; Vol. 123). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6890-9_15

Proteomic analysis of neuronal mitochondria. / Stauch, Kelly L.; Fox, Howard S.

Neuromethods. Humana Press Inc., 2017. p. 299-319 (Neuromethods; Vol. 123).

Research output: Chapter in Book/Report/Conference proceedingChapter

Stauch, KL & Fox, HS 2017, Proteomic analysis of neuronal mitochondria. in Neuromethods. Neuromethods, vol. 123, Humana Press Inc., pp. 299-319. https://doi.org/10.1007/978-1-4939-6890-9_15
Stauch KL, Fox HS. Proteomic analysis of neuronal mitochondria. In Neuromethods. Humana Press Inc. 2017. p. 299-319. (Neuromethods). https://doi.org/10.1007/978-1-4939-6890-9_15
Stauch, Kelly L. ; Fox, Howard S. / Proteomic analysis of neuronal mitochondria. Neuromethods. Humana Press Inc., 2017. pp. 299-319 (Neuromethods).
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