Proteomic analysis of early-responsive redox-sensitive proteins in Arabidopsis

Hai Wang, Shengbing Wang, Yuqing Lu, Sophie Alvarez, Leslie M. Hicks, Xiaochun Ge, Yiji Xia

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Regulation of protein function through oxidative modification has emerged as an important molecular mechanism modulating various biological processes. Here, we report a proteomic study of redox-sensitive proteins in Arabidopsis cells subjected to H 2O 2 treatment. Four gel-based approaches were employed, leading to the identification of four partially overlapping sets of proteins whose thiols underwent oxidative modification in the H 2O 2-treated cells. Using a method based on differential labeling of thiols followed by immunoprecipitation and Western blotting, five of the six selected putative redox-sensitive proteins were confirmed to undergo oxidative modification following the oxidant treatment in Arabidopsis leaves. Another method, which is based on differential labeling of thiols coupled with protein electrophoretic mobility shift assay, was adopted to reveal that one of the H 2O 2-sensitive proteins, a homologue of cytokine-induced apoptosis inhibitor 1 (AtCIAPIN1), also underwent oxidative modification in Arabidopsis leaves after treatments with salicylic acid or the peptide elicitor flg22, two inducers of defense signaling. The redox-sensitive proteins identified from the proteomic study are involved in various biological processes such as metabolism, the antioxidant system, protein biosynthesis and processing, and cytoskeleton organization. The identification of novel redox-sensitive proteins will be helpful toward understanding of cellular components or pathways previously unknown to be redox-regulated.

Original languageEnglish (US)
Pages (from-to)412-424
Number of pages13
JournalJournal of proteome research
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Arabidopsis Proteins
Proteomics
Oxidation-Reduction
Sulfhydryl Compounds
Proteins
Biological Phenomena
Arabidopsis
Labeling
Salicylic Acid
Protein Biosynthesis
Electrophoretic Mobility Shift Assay
Cytoskeleton
Immunoprecipitation
Electrophoretic mobility
Oxidants
Biosynthesis
Antioxidants
Metabolism
Western Blotting
Gels

Keywords

  • Arabidopsis
  • AtCIAPIN1
  • Redox proteomics
  • flg22
  • hydrogen peroxide
  • oxidative stress
  • salicylic acid

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Proteomic analysis of early-responsive redox-sensitive proteins in Arabidopsis. / Wang, Hai; Wang, Shengbing; Lu, Yuqing; Alvarez, Sophie; Hicks, Leslie M.; Ge, Xiaochun; Xia, Yiji.

In: Journal of proteome research, Vol. 11, No. 1, 01.01.2012, p. 412-424.

Research output: Contribution to journalArticle

Wang, Hai ; Wang, Shengbing ; Lu, Yuqing ; Alvarez, Sophie ; Hicks, Leslie M. ; Ge, Xiaochun ; Xia, Yiji. / Proteomic analysis of early-responsive redox-sensitive proteins in Arabidopsis. In: Journal of proteome research. 2012 ; Vol. 11, No. 1. pp. 412-424.
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