Proteomic analyses of monocytes obtained from Hispanic women with HIV-associated dementia show depressed antioxidants

Stephanie Kraft-Terry, Yamil Gerena, Valerie Wojna, Marines Plaud-Valentin, Yolanda Rodriguez, Pawel S Ciborowski, Raul Mayo, Richard Skolasky, Howard Eliot Gendelman, Loyda M. Meléndez

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: Monocyte ingress into the brain during progressive human immunodeficiency virus (HIV-1) infection parallels the severity of cognitive impairments. Although activated monocyte phenotypes emerge in disease, the functional correlates of these cells remain unresolved. Experimental design: To this end, we studied the proteome of blood-derived monocytes obtained from Hispanic women with the most severe form of HIV-associated neurocognitive disorders, HIV-associated dementia (HAD). Monocytes isolated from peripheral blood mononuclear cells by CD14+ immunoaffinity column chromatography were >95% pure. Cells were recovered from four patients without evidence of cognitive impairment and five with HAD and analyzed by 2-D DIGE and tandem MS. Results: Importantly, ADP ribosylhydrolase, myeloperoxidase, thioredoxin, peroxiredoxin 3, NADPH, and GTPase-activating protein were all downregulated in HAD. In regards to myeloperoxidase, thioredoxin, and peroxiredoxin 3, these changes were validated in an additional cohort of 30 patients by flow cytometry. Conclusions and clinical relevance: We conclude that deficits in monocyte antioxidants lead to neuronal damage through the loss of hydrogen peroxide scavenging capabilities; thus exposing neurons to apoptosis-inducing factors. Altered monocyte functions therefore may contribute to the development and progression of cognitive impairment.

Original languageEnglish (US)
Pages (from-to)706-714
Number of pages9
JournalProteomics - Clinical Applications
Volume4
Issue number8-9
DOIs
StatePublished - Sep 1 2010

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Peroxiredoxin III
AIDS Dementia Complex
Thioredoxins
ADP-ribosylarginine hydrolase
Hispanic Americans
Proteomics
Peroxidase
Monocytes
Blood
Antioxidants
Apoptosis Inducing Factor
GTPase-Activating Proteins
Column chromatography
Flow cytometry
Scavenging
Proteome
NADP
Viruses
Design of experiments
Hydrogen Peroxide

Keywords

  • 2-D-DIGE
  • HIV-associated dementia
  • HIV-associated neurocognitive disorders
  • MS
  • Monocytes

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Proteomic analyses of monocytes obtained from Hispanic women with HIV-associated dementia show depressed antioxidants. / Kraft-Terry, Stephanie; Gerena, Yamil; Wojna, Valerie; Plaud-Valentin, Marines; Rodriguez, Yolanda; Ciborowski, Pawel S; Mayo, Raul; Skolasky, Richard; Gendelman, Howard Eliot; Meléndez, Loyda M.

In: Proteomics - Clinical Applications, Vol. 4, No. 8-9, 01.09.2010, p. 706-714.

Research output: Contribution to journalArticle

Kraft-Terry, S, Gerena, Y, Wojna, V, Plaud-Valentin, M, Rodriguez, Y, Ciborowski, PS, Mayo, R, Skolasky, R, Gendelman, HE & Meléndez, LM 2010, 'Proteomic analyses of monocytes obtained from Hispanic women with HIV-associated dementia show depressed antioxidants', Proteomics - Clinical Applications, vol. 4, no. 8-9, pp. 706-714. https://doi.org/10.1002/prca.201000010
Kraft-Terry, Stephanie ; Gerena, Yamil ; Wojna, Valerie ; Plaud-Valentin, Marines ; Rodriguez, Yolanda ; Ciborowski, Pawel S ; Mayo, Raul ; Skolasky, Richard ; Gendelman, Howard Eliot ; Meléndez, Loyda M. / Proteomic analyses of monocytes obtained from Hispanic women with HIV-associated dementia show depressed antioxidants. In: Proteomics - Clinical Applications. 2010 ; Vol. 4, No. 8-9. pp. 706-714.
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