Protein tyrosine phosphatase inhibitors in FcγRI-induced myeloid oxidant signaling

Anat Erdreich-Epstein, Ming Liu, Yenbou Liu, Donald L. Durden

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Fc-receptor stimulation in myeloid cells results in increased oxygen consumption, termed the respiratory burst, which is coupled to a rapid and transient increase in tyrosine phosphorylation of cellular proteins. In a previous paper in this journal we showed that the protein tyrosine phosphatase (PTPase) inhibitors sodium orthovanadate and phenylarsine oxide (PAO) block the FcγRI-induced respiratory burst in interferon-γ- differentiated U937 cells (U937IF) while augmenting the FcγRI-induced tyrosine phosphorylation of cellular proteins. Herein we examine the effects of PTPase inhibitors on specific molecules involved in FcγRI signaling. We show that orthovanadate and PAO augmented the FcγRI-induced tyrosine phosphorylation of the adaptor protein CBL. CBL interactions with other phosphoproteins, among them SHC and CRKL, were also augmented in response to pretreatment with the PTPase inhibitors. SHC was tyrosine phosphorylated in response to FcγRI stimulation of U937IF cells and bound to the SH2 domain of GRB2 in a stimulation-dependent manner. In fusion protein pull-down experiments the interaction of SHC with the SH2 domain of GRB2 was increased in PTPase inhibitor pretreated U937IF cells in response to FcγRI stimulation. Our data support the hypothesis that a tyrosine dephosphorylation event is required for effective transmission of the FcγRI signal to result in activation of the myeloid respiratory burst response.

Original languageEnglish (US)
Pages (from-to)288-295
Number of pages8
JournalExperimental Cell Research
Volume237
Issue number2
DOIs
StatePublished - Dec 15 1997

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Protein Tyrosine Phosphatases
Oxidants
Tyrosine
Respiratory Burst
Vanadates
src Homology Domains
Phosphorylation
Proteins
U937 Cells
Fc Receptors
Phosphoproteins
Myeloid Cells
Oxygen Consumption
Interferons

ASJC Scopus subject areas

  • Cell Biology

Cite this

Protein tyrosine phosphatase inhibitors in FcγRI-induced myeloid oxidant signaling. / Erdreich-Epstein, Anat; Liu, Ming; Liu, Yenbou; Durden, Donald L.

In: Experimental Cell Research, Vol. 237, No. 2, 15.12.1997, p. 288-295.

Research output: Contribution to journalArticle

Erdreich-Epstein, Anat ; Liu, Ming ; Liu, Yenbou ; Durden, Donald L. / Protein tyrosine phosphatase inhibitors in FcγRI-induced myeloid oxidant signaling. In: Experimental Cell Research. 1997 ; Vol. 237, No. 2. pp. 288-295.
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