Protein-targeted corona phase molecular recognition

Gili Bisker, Juyao Dong, Hoyoung D. Park, Nicole M Iverson, Jiyoung Ahn, Justin T. Nelson, Markita P. Landry, Sebastian Kruss, Michael S. Strano

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by >80% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated by the quenching kinetics. The fibrinogen recognition also occurs in serum environment, at the clinically relevant fibrinogen concentrations in the human blood. These results open new avenues for synthetic, non-biological antibody analogues that recognize biological macromolecules, and hold great promise for medical and clinical applications.

Original languageEnglish (US)
Article number10241
JournalNature communications
Volume7
DOIs
StatePublished - Jan 8 2016

Fingerprint

fibrinogen
Molecular recognition
Fibrinogen
coronas
proteins
Carbon Nanotubes
Single-walled carbon nanotubes (SWCN)
Proteins
blood
Quenching
Fluorescence
carbon nanotubes
quenching
fluorescence
nodules
antibodies
Macromolecules
macromolecules
serums
Nanoparticles

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Bisker, G., Dong, J., Park, H. D., Iverson, N. M., Ahn, J., Nelson, J. T., ... Strano, M. S. (2016). Protein-targeted corona phase molecular recognition. Nature communications, 7, [10241]. https://doi.org/10.1038/ncomms10241

Protein-targeted corona phase molecular recognition. / Bisker, Gili; Dong, Juyao; Park, Hoyoung D.; Iverson, Nicole M; Ahn, Jiyoung; Nelson, Justin T.; Landry, Markita P.; Kruss, Sebastian; Strano, Michael S.

In: Nature communications, Vol. 7, 10241, 08.01.2016.

Research output: Contribution to journalArticle

Bisker, G, Dong, J, Park, HD, Iverson, NM, Ahn, J, Nelson, JT, Landry, MP, Kruss, S & Strano, MS 2016, 'Protein-targeted corona phase molecular recognition', Nature communications, vol. 7, 10241. https://doi.org/10.1038/ncomms10241
Bisker, Gili ; Dong, Juyao ; Park, Hoyoung D. ; Iverson, Nicole M ; Ahn, Jiyoung ; Nelson, Justin T. ; Landry, Markita P. ; Kruss, Sebastian ; Strano, Michael S. / Protein-targeted corona phase molecular recognition. In: Nature communications. 2016 ; Vol. 7.
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