Protein serine/threonine phosphotase-2A is differentially expressed and regulates eye development in vertebrates

W. B. Liu, X. H. Hu, X. W. Zhang, M. X. Deng, L. Nie, S. S. Hui, W. Duan, M. Tao, C. Zhang, J. Liu, W. F. Hu, Z. X. Huang, L. Li, M. Yi, T. T. Li, L. Wang, Y. Liu, S. J. Liu, D. W C Li

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Protein serine/threonine phosphatase-2A (PP-2A) is one of the key enzymes responsible for dephosphorylation in vertebrates. PP-2A-mediated dephosphorylation participates in many different biological processes including cell proliferation, differentiation, transformation, apoptosis, autophage and senescence. However, whether PP-2A directly controls animal development remains to be explored. Here, we present direct evidence to show that PP-2A displays important functions in regulating eye development of vertebrates. Using goldfish as a model system, we have demonstrated the following novel information. First, inhibition of PP-2A activity leads to significant death of the treated embryos, which is derived from blastomere apoptosis associated with enhanced phosphorylation of Bcl-XL at Ser-62, and the survived embryos displayed severe phenotype in the eye. Second, knockdown of PP-2A with morpholino oligomers leads to significant death of the injected embryos. The survived embryos from PP-2A knockdown displayed clear retardation in lens differentiation. Finally, overexpression of each catalytic subunit of PP-2A also causes death of majority of the injected embryos and leads to absence of goldfish eye lens or severely disturbed differentiation. Together, our results provide direct evidence that protein phosphatase-2A is important for normal eye development in goldfish.

Original languageEnglish (US)
Pages (from-to)1376-1384
Number of pages9
JournalCurrent Molecular Medicine
Volume13
Issue number8
DOIs
StatePublished - Sep 1 2013

Fingerprint

Threonine
Phosphoric Monoester Hydrolases
Serine
Vertebrates
Goldfish
Embryo Loss
Proteins
Embryonic Structures
Lenses
Apoptosis
Biological Phenomena
Protein Phosphatase 2
Blastomeres
Morpholinos
Crystalline Lens
Phosphorylation
Phosphoprotein Phosphatases
Cell proliferation
Oligomers
Cell Differentiation

Keywords

  • Apoptosis
  • Differentiation
  • Eye development
  • Lens
  • Pp-2a
  • Pp-2acoc/p
  • Protein phosphatases
  • Retina

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

Cite this

Protein serine/threonine phosphotase-2A is differentially expressed and regulates eye development in vertebrates. / Liu, W. B.; Hu, X. H.; Zhang, X. W.; Deng, M. X.; Nie, L.; Hui, S. S.; Duan, W.; Tao, M.; Zhang, C.; Liu, J.; Hu, W. F.; Huang, Z. X.; Li, L.; Yi, M.; Li, T. T.; Wang, L.; Liu, Y.; Liu, S. J.; Li, D. W C.

In: Current Molecular Medicine, Vol. 13, No. 8, 01.09.2013, p. 1376-1384.

Research output: Contribution to journalArticle

Liu, WB, Hu, XH, Zhang, XW, Deng, MX, Nie, L, Hui, SS, Duan, W, Tao, M, Zhang, C, Liu, J, Hu, WF, Huang, ZX, Li, L, Yi, M, Li, TT, Wang, L, Liu, Y, Liu, SJ & Li, DWC 2013, 'Protein serine/threonine phosphotase-2A is differentially expressed and regulates eye development in vertebrates', Current Molecular Medicine, vol. 13, no. 8, pp. 1376-1384. https://doi.org/10.2174/15665240113139990061
Liu, W. B. ; Hu, X. H. ; Zhang, X. W. ; Deng, M. X. ; Nie, L. ; Hui, S. S. ; Duan, W. ; Tao, M. ; Zhang, C. ; Liu, J. ; Hu, W. F. ; Huang, Z. X. ; Li, L. ; Yi, M. ; Li, T. T. ; Wang, L. ; Liu, Y. ; Liu, S. J. ; Li, D. W C. / Protein serine/threonine phosphotase-2A is differentially expressed and regulates eye development in vertebrates. In: Current Molecular Medicine. 2013 ; Vol. 13, No. 8. pp. 1376-1384.
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