Protein-protein interactions of tandem affinity purification-tagged protein kinases in rice

Jai S. Rohila, Mei Chen, Shuo Chen, Johann Chen, Ronald Cerny, Chris Dardick, Patrick Canlas, Xia Xu, Michael Gribskov, Siddhartha Kanrar, Jian Kang Zhu, Pamela Ronald, Michael E. Fromm

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Forty-one rice cDNAs encoding protein kinases were fused to the tandem affinity purification (TAP) tag and expressed in transgenic rice plants. The TAP-tagged kinases and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by mass spectrometry. Ninety-five percent of the TAP-tagged kinases were recovered. Fifty-six percent of the TAP-tagged kinases were found to interact with other rice proteins. A number of these interactions were consistent with known protein complexes found in other species, validating the TAP-tag method in rice plants. Phosphorylation sites were identified on four of the kinases that interacted with either 14-3-3 proteins or cyclins.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalPlant Journal
Volume46
Issue number1
DOIs
StatePublished - Apr 1 2006

Fingerprint

protein-protein interactions
protein kinases
Protein Kinases
phosphotransferases (kinases)
rice
Phosphotransferases
Genetically Modified Plants
Proteins
genetically modified organisms
rice protein
proteins
cyclins
14-3-3 Proteins
Cyclins
phosphorylation
mass spectrometry
Mass Spectrometry
Complementary DNA
Phosphorylation
Oryza

Keywords

  • Affinity
  • Oryza sativa
  • Protein kinase
  • Proteomics
  • Tandem affinity purification

ASJC Scopus subject areas

  • Plant Science

Cite this

Protein-protein interactions of tandem affinity purification-tagged protein kinases in rice. / Rohila, Jai S.; Chen, Mei; Chen, Shuo; Chen, Johann; Cerny, Ronald; Dardick, Chris; Canlas, Patrick; Xu, Xia; Gribskov, Michael; Kanrar, Siddhartha; Zhu, Jian Kang; Ronald, Pamela; Fromm, Michael E.

In: Plant Journal, Vol. 46, No. 1, 01.04.2006, p. 1-13.

Research output: Contribution to journalArticle

Rohila, JS, Chen, M, Chen, S, Chen, J, Cerny, R, Dardick, C, Canlas, P, Xu, X, Gribskov, M, Kanrar, S, Zhu, JK, Ronald, P & Fromm, ME 2006, 'Protein-protein interactions of tandem affinity purification-tagged protein kinases in rice', Plant Journal, vol. 46, no. 1, pp. 1-13. https://doi.org/10.1111/j.1365-313X.2006.02671.x
Rohila, Jai S. ; Chen, Mei ; Chen, Shuo ; Chen, Johann ; Cerny, Ronald ; Dardick, Chris ; Canlas, Patrick ; Xu, Xia ; Gribskov, Michael ; Kanrar, Siddhartha ; Zhu, Jian Kang ; Ronald, Pamela ; Fromm, Michael E. / Protein-protein interactions of tandem affinity purification-tagged protein kinases in rice. In: Plant Journal. 2006 ; Vol. 46, No. 1. pp. 1-13.
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AU - Canlas, Patrick

AU - Xu, Xia

AU - Gribskov, Michael

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