Protein phosphatase 2Cα expression in normal human tissues: An immunohistochemical study

Beatriz Lifschitz-Mercer, Yuri Sheinin, Daniella Ben-Meir, Letizia Bramante-Schreiber, Leonor Leider-Trejo, Shulamit Karby, Nechama I. Smorodinsky, Sara Lavi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Protein phosphatase (PP2Cα) is a member of the mammalian serine threonine-specific protein phosphatases family. We produced monoclonal antibodies against the recombinant PP2Cα and evaluated the immunoreactivity of normal human tissues. The reactivity was strong in normal skin, the digestive tract, lung, kidney, breast, prostate, endocrine glands, and brain, while it was moderate in the ovary, testis, and liver. Epithelial cells revealed high levels of PP2Cα expression, but stromal cells, including fibroblasts and endothelial cells, showed no or little PP2Cα expression. Given the broad reactivity in endocrine and secreting epithelial cells, we propose that PP2Cα expression might contribute to secretory cell function.

Original languageEnglish (US)
Pages (from-to)31-39
Number of pages9
JournalHistochemistry and Cell Biology
Volume116
Issue number1
StatePublished - Aug 11 2001

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Phosphoprotein Phosphatases
Epithelial Cells
Endocrine Glands
Stromal Cells
Gastrointestinal Tract
Testis
Prostate
Ovary
Breast
Endothelial Cells
Fibroblasts
Monoclonal Antibodies
Kidney
Lung
Skin
Liver
Brain
Protein Phosphatase 2C

Keywords

  • Histology
  • Human
  • Immunohistochemistry
  • Protein phosphatase 2Cα
  • Tissue distribution

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Lifschitz-Mercer, B., Sheinin, Y., Ben-Meir, D., Bramante-Schreiber, L., Leider-Trejo, L., Karby, S., ... Lavi, S. (2001). Protein phosphatase 2Cα expression in normal human tissues: An immunohistochemical study. Histochemistry and Cell Biology, 116(1), 31-39.

Protein phosphatase 2Cα expression in normal human tissues : An immunohistochemical study. / Lifschitz-Mercer, Beatriz; Sheinin, Yuri; Ben-Meir, Daniella; Bramante-Schreiber, Letizia; Leider-Trejo, Leonor; Karby, Shulamit; Smorodinsky, Nechama I.; Lavi, Sara.

In: Histochemistry and Cell Biology, Vol. 116, No. 1, 11.08.2001, p. 31-39.

Research output: Contribution to journalArticle

Lifschitz-Mercer, B, Sheinin, Y, Ben-Meir, D, Bramante-Schreiber, L, Leider-Trejo, L, Karby, S, Smorodinsky, NI & Lavi, S 2001, 'Protein phosphatase 2Cα expression in normal human tissues: An immunohistochemical study', Histochemistry and Cell Biology, vol. 116, no. 1, pp. 31-39.
Lifschitz-Mercer B, Sheinin Y, Ben-Meir D, Bramante-Schreiber L, Leider-Trejo L, Karby S et al. Protein phosphatase 2Cα expression in normal human tissues: An immunohistochemical study. Histochemistry and Cell Biology. 2001 Aug 11;116(1):31-39.
Lifschitz-Mercer, Beatriz ; Sheinin, Yuri ; Ben-Meir, Daniella ; Bramante-Schreiber, Letizia ; Leider-Trejo, Leonor ; Karby, Shulamit ; Smorodinsky, Nechama I. ; Lavi, Sara. / Protein phosphatase 2Cα expression in normal human tissues : An immunohistochemical study. In: Histochemistry and Cell Biology. 2001 ; Vol. 116, No. 1. pp. 31-39.
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