Protein kinase inhibitors modulate expression of alpha-ib adrenergic receptors in transfected chinese hamster ovary (cho) cells

K. S. Bird, S. J. Zhu, J. L. Anderson, Myron Lee Toews

Research output: Contribution to journalArticle

Abstract

Epinephrine (EPI) induced an unexpected up-regulation of [3H]prazosin binding to intact cells in a clone (H99) of CHO cells stably transfected to express high levels of the hamster alpha-1B adrenergic receptor. The protein kinase C inhibitor staurosporine (ST) also induced up-regulation. Exposure to 10 nM ST for 24 hr increased both the BnMX and KD values by approximately 2-fold, similar to the effects of EPI. Up-regulation occurred steadily over the 24 hr period, with half-maximal up-regulation at 1 nM ST. Neither the protein kinase C inhibitor GF109203X nor the tyrosine kinase inhibitor genistein induced up-regulation. However, genistein but not GF109203X inhibited EPI-induced upregulation; the less active genistein analog daidzein inhibited EPI up-regulation at higher concentrations. The protein synthesis inhibitor cycloheximide at low concentrations blocked ST-induced up-regulation and at higher concentrations revealed ST-induced down-regulation. In a sister clone (H88) in which EPI induced down-regulation, ST induced down-regulation as well. These results suggest multiple roles for protein kinases in regulation of transfected receptor expression.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

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Receptors, Adrenergic, alpha
Staurosporine
Chinese hamsters
Protein Kinase Inhibitors
Cricetulus
protein kinases
Adrenergic Receptors
epinephrine
Ovary
Up-Regulation
Epinephrine
Cells
Genistein
genistein
protein kinase C
cells
Protein Kinase C
Down-Regulation
Protein C Inhibitor
Adrenergic alpha-1 Receptors

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Protein kinase inhibitors modulate expression of alpha-ib adrenergic receptors in transfected chinese hamster ovary (cho) cells. / Bird, K. S.; Zhu, S. J.; Anderson, J. L.; Toews, Myron Lee.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

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