Protein kinase C expression in salivary gland acinar epithelial cells in non-obese diabetic mice, an experimental model for Sjögren's syndrome.

E. K. Tensing, J. Ma, M. Hukkanen, Howard S Fox, T. F. Li, J. Törnwall, Y. T. Konttinen

Research output: Contribution to journalArticle

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Abstract

We planned to investigate the expression of protein kinase C (PKC) isoforms in acinar epithelial cells of salivary glands in the non-obese diabetic (NOD) mouse to find out if they develop changes of the PKC system like those seen in the human counterpart, i.e. in Sjögren's syndrome. Parotid, submandibular, and sublingual glands from NOD and control BALB/c mice were stained with a panel of monoclonal antibodies directed against conventional (alpha, beta, and gamma), novel (delta, epsilon, and theta), and atypical (lambda and iota) PKC isoforms using the streptavidin/HRP method. Similarly to human labial salivary glands, acinar epithelial cells of the healthy control BALB/c mice contained two of the conventional PKC isoforms, alpha and beta. Acinar and ductal epithelial cells also contained the atypical PKC isoforms lambda and iota. PKC isoforms gamma, delta, epsilon, and theta were not found. NOD mice which displayed focal sialadenitis contained the same conventional and atypical PKC isoforms. The acinar cells in NOD mice, in contrast to the Sjögren's syndrome patients, did not lack PKC alpha or beta. On the contrary, PKC alpha and beta staining was stronger than in the control BALB/c mice. The present results demonstrate that both conventional and atypical PKC isoforms participate in the salivary epithelial cell biology and that there are mouse strain-associated and/or disease state-associated changes in their expression. The lack of PKC alpha and beta isoforms found in Sjögren's syndrome was not reproduced in NOD mice, which discloses one more difference between the human disease and its NOD mouse model.

Original languageEnglish (US)
Pages (from-to)28-32
Number of pages5
JournalRheumatology international
Volume25
Issue number1
DOIs
StatePublished - Jan 1 2005

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Inbred NOD Mouse
Acinar Cells
Salivary Glands
Protein Kinase C
Protein Isoforms
Theoretical Models
Epithelial Cells
Protein Kinase C beta
Protein Kinase C-alpha
Sublingual Gland
Sialadenitis
Streptavidin
Submandibular Gland
Parotid Gland
Lip
Cell Biology
Monoclonal Antibodies
Staining and Labeling
protein kinase C lambda

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

Cite this

Protein kinase C expression in salivary gland acinar epithelial cells in non-obese diabetic mice, an experimental model for Sjögren's syndrome. / Tensing, E. K.; Ma, J.; Hukkanen, M.; Fox, Howard S; Li, T. F.; Törnwall, J.; Konttinen, Y. T.

In: Rheumatology international, Vol. 25, No. 1, 01.01.2005, p. 28-32.

Research output: Contribution to journalArticle

Tensing, E. K. ; Ma, J. ; Hukkanen, M. ; Fox, Howard S ; Li, T. F. ; Törnwall, J. ; Konttinen, Y. T. / Protein kinase C expression in salivary gland acinar epithelial cells in non-obese diabetic mice, an experimental model for Sjögren's syndrome. In: Rheumatology international. 2005 ; Vol. 25, No. 1. pp. 28-32.
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